A chemokine-to-cytokine-to-chemokine cascade critical in antiviral defense
J. Clin. Invest. Thais P. Salazar-Mather, et al. 105:985 doi:10.1172/JCI9232 [Go to this article.]

Figure 4
Requirements for IFN-γ and Mig gene expression. (a and b) Liver RNA was isolated from MIP-1α⁺, MIP-1α-, or IFN-γ^– mice that were uninfected or infected with MCMV. (a) Total RNA was analyzed by Northern blot hybridization. Results represent 1 of 3 experiments. (b) Relative quantitative RT-PCR was carried out with Competimers for 18-S rRNA as internal controls. (c) Total liver RNA was prepared from MIP-1α⁺ and MIP-1α^– mice administered control IgG (denoted as C) or anti-CD3 (α-CD3) at 3 hours before harvest. RNA was prepared and analyzed by relative quantitative RT-PCR as above. (d) Liver RNA was isolated from mice that were uninfected or infected with MCMV, with or without NK cell depletions. Mice were C57BL/6 or C57BL/6-SCID mice treated with control IgG (denoted as C), anti-NK1.1, or anti-AGM1 24 hours before infection. RNA was analyzed by relative quantitative RT-PCR as above. b–d show ethidium bromide–stained gel of amplified products, representing 1 of 3 experiments (see Table 1 for quantitation of results and Methods for details of procedures).