T cell–mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis
J. Clin. Invest. Axel Trautmann, et al. 106:25 doi:10.1172/JCI9199 [Go to this article.]

Figure 4
Induction of KC apoptosis by type 1 and type 2 T cells. (a) Type 1 but not type 2 T cells mediated KC death. Coculture of primary human KCs and heterologous differentiated type 1 and type 2 T cells. ^AP < 0.05. (b) Cytokine content of differentiated and stimulated type 2 T cells as determined by ELISA. CD4⁺ T helper 2 (Th2) and CD8⁺ T cytotoxic 2 (Tc2) cells were stimulated with anti-CD2, anti-CD3, and anti-CD28 mAb’s or with a combination of anti-CD2, anti-CD3, and anti-CD28 mAb’s and IL-12. ^AP < 0.05. (c) Coculture of primary human KCs and heterologous differentiated type 2 T cells. KC viability was measured by ethidium bromide exclusion and flow cytometry at day 3. Control, KCs alone. CD4⁺ Th2 cells and CD8⁺ Tc2 cells were stimulated with anti-CD2, anti-CD3, and anti-CD28 mAb’s or with a combination of anti-CD2, anti-CD3, and anti-CD28 mAb’s and IL-12. ^AP < 0.05. (d) Levels of soluble FasL in T-cell supernatants as determined by ELISA. CD4⁺ Th1/Th2 and CD8⁺ Tc1/Tc2 cells were stimulated with anti-CD2, anti-CD3, and anti-CD28 mAb’s or with a combination of anti-CD2, anti-CD3, and anti-CD28 mAb’s and IL-12. Results shown represent three (a, c) to five (b, d) experiments and are shown as mean ± SD from triplicate cultures.