Human allograft acceptance is associated with immune regulation
J. Clin. Invest. A.M. VanBuskirk, et al. 106:145 doi:10.1172/JCI9171 [Go to this article.]

Figure 3
Effect of cytokine neutralization on donor-reactive DTH. (a) Eight million PBMCs from patient D.S. were mixed with 10 μg donor or self antigen or 10⁵ irradiated donor-derived EBV-transformed LCL and 25 μg of either control rabbit IgG or rabbit anti-human TGF-β IgG and injected into the footpads of Balb/c SCID mice. Swelling was measured after 24 hours. Results are expressed as the change over the swelling induced by injection of PBMCs alone. To determine the effect of neutralizing antibodies to IL-10, 8 million PBMCs from patient D.S. were mixed with 10 μg donor antigen or 10⁵ irradiated donor-derived EBV-transformed LCL and 25 μg of either control goat IgG or goat anti-human IL-10. Swelling was measured after 24 hours, as described above. (b) Eight million PBMCs from patient A.J. were mixed with 10 μg donor antigens and 25 μg of either control rabbit IgG or rabbit anti-human TGF-β IgG and injected into the footpads of SCID mice. Swelling was measured as described above. To assess the effect of neutralizing antibodies to IL-10, PBMCs from patient A.J. were mixed with donor or self antigens and 25 μg of either control Ig or anti–IL-10. The results shown are from three separate experiments. In two experiments, the anti–IL-10 was a mAb with a mouse IgG-1 control. In the third experiment, goat anti-human IL-10 and goat IgG were used as the specific antibody and control, respectively. The results were not significantly different whether the polyclonal antibody or mAb was used.