Human allograft acceptance is associated with immune regulation
J. Clin. Invest. A.M. VanBuskirk, et al. 106:145 doi:10.1172/JCI9171 [Go to this article.]

Figure 2
Specificity analysis of donor antigen–linked DTH nonresponsiveness. (a) Eight million PBMCs from patient D.S. were injected into the footpads of Balb/c SCID mice either alone or mixed with 10 μg EBV lysate or 10⁵ autologous or donor-derived EBV-transformed LCL. Swelling was measured after 24 hours and is expressed as the change over the swelling induced by injection of PBMCs alone. Injection of EBV lysate or EBV-transformed LCL alone did not result in any increase in footpad thickness. The PBMCs also did not respond to control lysate prepared from Raji cells (not shown). (b) Eight million PBMCs from patient D.S. were injected into the footpads either alone or mixed with 10⁵ irradiated 721.221, A2-.221, or B62-.221 LCL. Swelling was measured as described above. (c) Eight million PBMCs from patient D.S. were mixed with TT or TT plus purified HLA-A2 or HLA-B62 and injected into the footpads of SCID mice. Swelling was measured after 24 hours as described above.