Effect of intraperitoneally and topically supplemented leptin on blood glucose level, body weight, and wound healing in ob/ob mice. C57BL/6J-ob/ob mice were treated with leptin intraperitoneally (i.p.) or topically (top) as described in Methods. PBS-treated ob/ob mice were used as a control. (a and b) Blood glucose levels and body weight were determined for intraperitoneally injected (a) or topically treated (b) animals at the indicated experimental time points. Three animals (n = 3) were analyzed at every experimental time point. Data are expressed as milligrams per deciliter (for blood glucose levels) or grams (for body weight). Mean changes ± SD in blood glucose levels, or body weight, respectively, are shown. ^AP < 0.05; ^BP < 0.01; NS, not significant compared with the conditions as indicated with the brackets. (c) Thirteen-day wounds after intraperitoneal (i.p.) treatment of animals with 5 μg leptin/g of body weight (+leptin/i.p.) or PBS (+PBS/i.p.). (d) Back skin of representative C57BL/6J-ob/ob mice topically treated with 1 μg leptin in 20 μL PBS twice a day (+leptin/top.) or PBS (+PBS/top.) at day 10 after wounding. The bottom panels demonstrate the same wounds in higher magnification.