C-C chemokine–encoding DNA vaccines enhance breakdown of tolerance to their gene products and treat ongoing adjuvant arthritis
J. Clin. Invest. Sawsan Youssef, et al. 106:361 doi:10.1172/JCI9109 [Go to this article.]

Figure 3
Breakdown of tolerance to self in DNA-vaccinated rats leads to the production of self-specific Ab’s. Lewis rats were subjected to the subsequent administration of various C-C chemokine DNA constructs, as described in Figure 1. Control rats were injected with pcDNA3 alone or with PBS. Three weeks later these rats were separated to subgroups that were administered CFA in two different ways: footpad injection to induce a local DTH response and tail-base administration to induce poly-arthritis. On day 20 anti-self Ab titers in serum were determined by ELISA. Results are shown as mean of three different serum samples ± SE.