Deactivation of peroxisome proliferator–activated receptor-α during cardiac hypertrophic growth
J. Clin. Invest. Philip M. Barger, et al. 105:1723 doi:10.1172/JCI9056 [
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Figure 5Downregulation of PPAR-α and FAO enzyme gene expression in the pressure overload–induced hypertrophied mouse heart. Representative autoradiograph of a Northern blot analysis performed with total RNA isolated from the left ventricles of mice 7 days after placement of a constricting band around the transverse aortic arch (B) or sham operation (S). Ten micrograms of RNA was loaded per lane. The blot was sequentially hybridized with the radiolabeled cDNA probes indicated at left (described under Methods). 18S ribosomal RNA stained with ethidium bromide and visualized by UV fluorescence is shown as a loading control. This figure is representative of the results obtained across four pairs of age-matched, banded (
n = 7) mice and sham-operated control (
n = 7) littermates (aged 3–5 months).
