Deactivation of peroxisome proliferator–activated receptor-α during cardiac hypertrophic growth
J. Clin. Invest. Philip M. Barger, et al. 105:1723 doi:10.1172/JCI9056 [Go to this article.]

Figure 2
Basal and fatty acid–activated M-CPT I gene expression is repressed during cardiac myocyte hypertrophy. (a) Representative autoradiograph of Northern blot analyses performed with total RNA isolated from rat neonatal cardiac myocytes in culture. Each lane contained 10 μg of total RNA isolated from cardiac myocytes incubated in the presence of 100 μM PE, 250 μM oleate complexed to BSA, or vehicle control (water, BSA, or both). The blot was sequentially hybridized with radiolabeled cDNA probes encoding M-CPT I or β-actin. (b) Bars represent mean (± SEM) steady-state M-CPT I mRNA levels as determined by phosphorimage analysis of bands on Northern blots of RNA obtained from at least four separate experiments. Values shown are arbitrary units corrected to actin signal intensity and normalized (= 1.0) to the value obtained with vehicle alone. ^ASignificantly different (P < 0.05; ANOVA coupled to Scheffe test) from the values obtained from samples prepared from cells exposed to vehicle alone. ^BSignificantly different from values obtained with cells treated with oleate alone.