JCI - Insulin receptor substrate-1 in osteoblast is indispensable for maintaining bone turnover

Insulin receptor substrate-1 in osteoblast is indispensable for maintaining bone turnover

Naoshi Ogata, Daichi Chikazu, Naoto Kubota, Yasuo Terauchi, Kazuyuki Tobe, Yoshiaki Azuma, Tomohiro Ohta, Takashi Kadowaki, Kozo Nakamura, Hiroshi Kawaguchi

Published in Volume 105, Issue 7

J Clin Invest. 2000; 105(7):935–943 doi:10.1172/JCI9017

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Figure 4

Cultures of osteoblasts from WT and IRS-1^–/– mice. (a) Cell proliferation of cultured osteoblasts. Osteoblasts from neonatal calvariae of WT and IRS-1^–/– littermates were cultured with or without IGF-I (10 nM), insulin (100 nM), or FGF-2 (1 nM) for 24 hours, and the cell proliferation was determined by [³H]-TdR incorporation into DNA. (b) ALP activity of osteoblasts from WT or IRS-1^–/– neonatal calvariae cultured with or without IGF-I (10 nM), insulin (100 nM), or BMP-2 (30 ng/mL) at 14 days of culture. Data are expressed as mean (bars) ± SEM (error bars) for 8 wells/group. Significantly different from WT cultures, ^AP < 0.05, ^BP < 0.01.