JCI - Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Aldemar Montero, Yosuke Okada, Masato Tomita, Masako Ito, Hiroshi Tsurukami, Toshitaka Nakamura, Thomas Doetschman, J. Douglas Coffin, Marja M. Hurley

Published in Volume 105, Issue 8

J Clin Invest. 2000; 105(8):1085–1093 doi:10.1172/JCI8641

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Figure 2

Comparison of thymidine incorporation into DNA in calvarial osteoblasts from 8-week-old Fgf2^+/+ and Fgf2^–/– mice. Osteoblastic cells were prepared from calvariae of 8-week-old Fgf2^+/+ and Fgf2^–/– mice and plated in 100-mm dishes in DMEM with 10% heat inactivated FCS for 12 days. Cells were harvested and replated at a density of 5,000 cells/cm² for 7 days. Media were changed every 3 days. Cultures were treated with fresh media in the absence or presence of FGF-2 (10 nM) for the last 24 hours of the culture period. For labeling studies, [³H]thymidine (10 μCi/well) was added for the last 4 hours of the culture to measure thymidine incorporation into DNA. Values are the mean ± SEM for 6 determinations per group. ^ASignificantly different from control cultures; P < 0.05. ^BSignificantly different from Fgf2^+/+; P < 0.05.