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Natural antibodies with the T15 idiotype may act in atherosclerosis, apoptotic clearance, and protective immunity
Peter X. Shaw, Sohvi Hörkkö, Mi-Kyung Chang, Linda K. Curtiss, Wulf Palinski, Gregg J. Silverman, Joseph L. Witztum
Published in Volume 105, Issue 12
J Clin Invest. 2000; 105(12):1731–1740 doi:10.1172/JCI8472
This article Copyright © 2000, The American Society for Clinical Investigation
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Figure 5

Binding of EO6 and T15 to pneumococcal C-PS. (a) C-PS was plated on microtiter wells at the indicated concentrations overnight at 4°C. Each Ab (5 μg/mL) was added to the plate and detected by AP-conjugated secondary Abs (goat anti-mouse IgM for EO6 and EO14, goat anti-mouse IgA for T15). The amount of bound Ab was expressed as RLU/100 ms. (b) Cu-OxLDL or MDA-LDL (10 μg/mL) was plated as antigen, and the indicated Ab was added to microtiter wells in the absence or presence of the indicated concentrations of C-PS. Bound Ab was detected by AP-conjugated secondary Abs (goat anti-mouse IgM for EO6 and EO14, goat anti-mouse IgA for T15), and was expressed as percent control of Ab binding to its antigen without competitor.

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