Albuterol-induced downregulation of Gsα accounts for pulmonary β₂-adrenoceptor desensitization in vivo
J. Clin. Invest. Paul A. Finney, et al. 106:125 doi:10.1172/JCI8374 [Go to this article.]

Figure 7
Effect of chronic treatment of rats with albuterol on cAMP PDE activity in lung. The ability of rolipram (30 μM; a), Org 9935 (30 μM; b), and a combination of rolipram and Org 9935 (c) to suppress cAMP hydrolysis was assessed in lung taken from rats treated chronically with saline and albuterol. Open and filled bars show PDE activity in the absence and presence of PDE inhibitor, respectively. In each panel, B/A corresponds to the fold-increase in activity attributable to the PDE isoenzyme(s) defined with rolipram, Org 9935, or rolipram/Org 9935. Total induction is given by (B + C + D)/(A + D). Data represent the mean ± SEM of eight determinations. ^EP < 0.05, significant inhibition of PDE activity.