Albuterol-induced downregulation of Gsα accounts for pulmonary β₂-adrenoceptor desensitization in vivo
J. Clin. Invest. Paul A. Finney, et al. 106:125 doi:10.1172/JCI8374 [Go to this article.]

Figure 6
Effect of chronic treatment of rats with albuterol on Gsα expression in lung membranes. Rats were given saline (filled bars) or albuterol (gray bars; 40 μg/kg/h) for 7 days, and the expression of membrane-associated Gsα was determined. (a) Representative Western blot and bar charts of the mean (± SEM) results of nine determinations. (b) Representative digitized phosphorimage of the amount of Gsα assessed by CTX-catalyzed NAD⁺-dependent ADP ribosylation. Equal loading was confirmed by staining of the gels with Coomassie blue. Sal, animals treated with saline; Alb, animals treated with albuterol. ^AP < 0.05, significant reduction in Gsα expression.