JCI - Altered function of insulin receptor substrate-1–deficient mouse islets and cultured β-cell lines

Altered function of insulin receptor substrate-1–deficient mouse islets and cultured β-cell lines

Rohit N. Kulkarni, Jonathon N. Winnay, Molly Daniels, Jens C. Brüning, Sarah N. Flier, Douglas Hanahan, C. Ronald Kahn

J Clin Invest. 1999; 104(12):R69–R75 doi:10.1172/JCI8339

Abstract | Full text | PDF

Options: View larger image (or click on image)

Figure 6

Insulin content is partially restored in IRS-1 re-expressing cultured β-cells. (a) A reduced insulin content is observed in IRS-1^–/– and IRS-1^+/– cells compared with WT cells. Re-expression of IRS-1 in IRS-1^–/– cells (upper panel) shows a partial restoration of insulin content and is significantly higher than IRS-1^–/– cells (lower panel). Values are means ± SEM (n = 6–7). *P < 0.05 versus WT; **P < 0.01 versus WT; #P < 0.05 versus IRS-1^–/–. Insulin secretion is blunted in response to increasing concentrations of (b) glucose and (c) L-arginine in IRS-1^–/– and IRS-1^+/– β-cell lines. Results are expressed as a percent of the cellular insulin content. Values are means ± SEM (n = 6). P < 0.05 IRS-1^+/– or IRS-1^–/– versus WT at all concentrations; P < 0.05 IRS-1^+/– versus IRS-1^–/– at all concentrations except 0.01 and 0.1 mM glucose and 0.1 and 5.5 mM L-arginine.