Targeted expression of a dominant-negative EGF-R in the kidney reduces tubulo-interstitial lesions after renal injury
J. Clin. Invest. Fabiola Terzi, et al. 106:225 doi:10.1172/JCI8315 [Go to this article.]

Figure 2
Transgene function. (a and b) Western blot analysis of EGF-R autophosphorylation in transgenic (EGF-R-M) and wild-type (WT) mice. (a) Transgene expression reduced the phosphorylation of EGF-R in transgenic mice after EGF injection. Proteins were extracted from whole kidney and immunoprecipitated with an anti–EGF-R antibody. The immunoprecipitate was probed with the RC-20 anti-phosphotyrosine antibody. (b) Increasing amounts (50, 100, 200, and 300 μg) of proteins were immunoprecipitated to validate the method used to quantify EGF-R phosphorylation (linear regression: r² = 1.00 and 0.985 for wild-type and transgenic mice, respectively). (c) Binding curves and Scatchard plots (inset) of ¹²⁵I-EGF binding. Transgene expression abolished the high-affinity state of the endogenous receptor in isolated proximal tubules. Note that different scales were used in the two insets.