Targeted expression of a dominant-negative EGF-R in the kidney reduces tubulo-interstitial lesions after renal injury
J. Clin. Invest. Fabiola Terzi, et al. 106:225 doi:10.1172/JCI8315 [Go to this article.]

Figure 1
Transgene expression and function in transgenic mice. (a) Northern blot analysis. The transgene was detected exclusively in the kidney using the human β-globin poly(A) probe. (b) Immunohistochemical analysis with a specific anti-human EGF-R antibody. The transgene was localized in basolateral membranes of proximal transgenic tubules (EGF-R-M, middle panel), but not in those of wild-type membranes (WT, left panel). Specific staining was detected in the cortex (C) but not in the medulla (M) of transgenic mice (right panel). (c) Western blot analysis of mouse (m-EGF-R) and human (h-EGF-R) EGF receptors in proximal tubular cells from WT and EGF-R-M mice. Similar amounts of endogenous EGF-R were detected by a specific anti–EGF-R intracellular domain antibody in EGF-R-M and WT mice (m-EGF-R), whereas a 110-kDa protein, corresponding to the truncated receptor, was evidenced in transgenic but not in wild-type mice, by a specific anti-human EGF-R antibody (h-EGF-R). A431 cells overexpressing the human endogenous EGF-R (h-EGF-R, 170 kDa) was used as positive control.