Gly369Cys mutation in mouse FGFR3 causes achondroplasia by affecting both chondrogenesis and osteogenesis
J. Clin. Invest. Lin Chen, et al. 104:1517 doi:10.1172/JCI6690 [Go to this article.]

Figure 5
Histology and immunohistochemistry analyses of growth plates of P15 mice. Genotypes and markers were as indicated. (a) Chondrocytes in the WT growth plates are divided into 4 distinct zones, i.e. resting (Rc), proliferating (Pc), maturing (Mc) and hypertrophic (Hc) chondrocytes. (b and c) In the mutant growth plates, the demarcation of each zone is not clear. Arrows point to the secondary ossification center. (d–f) [³H]Thymidine incorporation. The labeled cells in WT mark the Pc zone. In mutant growth plates, the labeled cells are fewer in number and scattered, suggesting that the majority of the cells are in a quiescent state. (g–o) Immunohistochemical analysis of growth plates, using antibodies to FGFR3 (g–i), Stat5b (j–l, and m–o for higher magnification), and p19 (p–r). In all cases, the staining was the weakest in WT, intermediate in 369/+, and strongest in 369/369 growth plates.