Gly369Cys mutation in mouse FGFR3 causes achondroplasia by affecting both chondrogenesis and osteogenesis
J. Clin. Invest. Lin Chen, et al. 104:1517 doi:10.1172/JCI6690 [Go to this article.]

Figure 1
Introduction of Gly369Cys mutation into the mouse Fgfr3 locus. (a) Targeting construct, pFgfr3-Gly369Cys, contained the Gly369Cys mutation in exon 10 and a pLoxpneo gene in intron 10 of the Fgfr3 gene. Of 120 G418^r/FIAU^r clones examined by Southern blot analysis using a 5′ flanking probe (probe 1), 5 clones showed an extra fragment of approximately 11 kb upon NotI + EcoRV digestion (c). The targeting events were confirmed by XbaI +EcoRV digestion using a 3′ internal probe (probe 2). Three of them had the point mutation co-transferred as indicated by sequencing (unpublished observations). (b) Removal of the pLoxpneo gene by breeding with EIIa-cre transgenic mice. The Cre-mediated deletion was genotyped by PCR as described in the Methods section.