Michael Didié, Peter Christalla, Michael Rubart, Vijayakumar Muppala, Stephan Döker, Bernhard Unsöld, Ali El-Armouche, Thomas Rau, Thomas Eschenhagen, Alexander P. Schwoerer, Heimo Ehmke, Udo Schumacher, Sigrid Fuchs, Claudia Lange, Alexander Becker, Wen Tao, John A. Scherschel, Mark H. Soonpaa, Tao Yang, Qiong Lin, Martin Zenke, Dong-Wook Han, Hans R. Schöler, Cornelia Rudolph, Doris Steinemann, Brigitte Schlegelberger, Steve Kattman, Alec Witty, Gordon Keller, Loren J. Field, Wolfram-Hubertus Zimmermann
Proof-of-concept for the therapeutic application of MHC-matched EHM grafts.
(A) F-EHM graft sutured onto a beating heart. (B) EHM graft on infarcted myocardium (PCMs, EGFP-positive; heart was explanted directly after EHM engraftment for demonstration). (C) Anterior wall thickness in diastole (AWThd) and (D) anterior wall thickening fraction (AWThF) measured by echocardiography 2 weeks after MI and the respective surgical intervention. Sham, no MI, and no graft; control (Ctrl), MI with no graft; F-EHM, MI with formaldehyde-fixed (nonviable) EHM; EHM, MI with viable EHM graft (n = 7–8). Data represent means ± SEM; *P < 0.05 versus control (also refer to Supplemental Table 3). (E) Heart explants with F-EHM and EHM (EGFP-positive) grafts; EGFP signal (lower panels) indicates retention of viable cardiomyocytes. (F) Anisotropically arranged PCMs (α-actinin and GFP) in EHM graft. (G) PCMs surrounded by CD31-positive capillaries. Scale bars: (A, B, and E) 2 mm; (F and G) 20 μm.