J Clin Invest.
Proof-of-concept for the therapeutic application of MHC-matched EHM grafts.
(A) F-EHM graft sutured onto a beating heart. (B) EHM graft on infarcted myocardium (PCMs, EGFP-positive; heart was explanted directly after EHM engraftment for demonstration). (C) Anterior wall thickness in diastole (AWThd) and (D) anterior wall thickening fraction (AWThF) measured by echocardiography 2 weeks after MI and the respective surgical intervention. Sham, no MI, and no graft; control (Ctrl), MI with no graft; F-EHM, MI with formaldehyde-fixed (nonviable) EHM; EHM, MI with viable EHM graft (n = 7–8). Data represent means ± SEM; *P < 0.05 versus control (also refer to Supplemental Table 3). (E) Heart explants with F-EHM and EHM (EGFP-positive) grafts; EGFP signal (lower panels) indicates retention of viable cardiomyocytes. (F) Anisotropically arranged PCMs (α-actinin and GFP) in EHM graft. (G) PCMs surrounded by CD31-positive capillaries. Scale bars: (A, B, and E) 2 mm; (F and G) 20 μm.