Michael Didié, Peter Christalla, Michael Rubart, Vijayakumar Muppala, Stephan Döker, Bernhard Unsöld, Ali El-Armouche, Thomas Rau, Thomas Eschenhagen, Alexander P. Schwoerer, Heimo Ehmke, Udo Schumacher, Sigrid Fuchs, Claudia Lange, Alexander Becker, Wen Tao, John A. Scherschel, Mark H. Soonpaa, Tao Yang, Qiong Lin, Martin Zenke, Dong-Wook Han, Hans R. Schöler, Cornelia Rudolph, Doris Steinemann, Brigitte Schlegelberger, Steve Kattman, Alec Witty, Gordon Keller, Loren J. Field, Wolfram-Hubertus Zimmermann
Tissue-engineered myocardium from PSC derivatives.
(A) Light microscopic (top panel) and epifluorescence images (bottom panel) of an EHM (culture day 8; also refer to Supplemental Video 2). (B) Confocal laser scanning microscopy of a cardiomyocyte bundle in EHM. EGFP (green); α-actinin (red); nuclei (blue, DAPI). (C) Transcript abundance of Myh6 and Myh7 (normalized to Casq2) in native myocardium (fetal, neonatal, adult), EB cultures (ESC and PSC), and EHM (n = 3–4 per group; data represent means ± SEM). (D) Concentration response curve under increasing extracellular [Ca2+] for EHM (n = 8) and native mouse myocardium (ventricular wedge; n = 4). Data represent means ± SEM (also refer to Supplemental Table 2). Single isometric contractions of EHM and native heart muscle (inset) at low (0.4 mmol/l) and high (2.4 mmol/l) extracellular [Ca2+]. Scale bars: (A) 1 mm, (B) 50 μm.