J Clin Invest.
Tissue-engineered myocardium from PSC derivatives.
(A) Light microscopic (top panel) and epifluorescence images (bottom panel) of an EHM (culture day 8; also refer to Supplemental Video 2). (B) Confocal laser scanning microscopy of a cardiomyocyte bundle in EHM. EGFP (green); α-actinin (red); nuclei (blue, DAPI). (C) Transcript abundance of Myh6 and Myh7 (normalized to Casq2) in native myocardium (fetal, neonatal, adult), EB cultures (ESC and PSC), and EHM (n = 3–4 per group; data represent means ± SEM). (D) Concentration response curve under increasing extracellular [Ca2+] for EHM (n = 8) and native mouse myocardium (ventricular wedge; n = 4). Data represent means ± SEM (also refer to Supplemental Table 2). Single isometric contractions of EHM and native heart muscle (inset) at low (0.4 mmol/l) and high (2.4 mmol/l) extracellular [Ca2+]. Scale bars: (A) 1 mm, (B) 50 μm.