Michael Didié, Peter Christalla, Michael Rubart, Vijayakumar Muppala, Stephan Döker, Bernhard Unsöld, Ali El-Armouche, Thomas Rau, Thomas Eschenhagen, Alexander P. Schwoerer, Heimo Ehmke, Udo Schumacher, Sigrid Fuchs, Claudia Lange, Alexander Becker, Wen Tao, John A. Scherschel, Mark H. Soonpaa, Tao Yang, Qiong Lin, Martin Zenke, Dong-Wook Han, Hans R. Schöler, Cornelia Rudolph, Doris Steinemann, Brigitte Schlegelberger, Steve Kattman, Alec Witty, Gordon Keller, Loren J. Field, Wolfram-Hubertus Zimmermann
Cardiomyocyte differentiation from PSCs in vitro and in vivo.
(A) FACS of GFP-positive PCMs (culture day 7 + 15); nontransgenic ESCs served as the control cell population (right panel illustrates a PCM 24 hours after plating; also refer to Supplemental Video 1). (B) Morphology of FACS-purified PCMs (EGP-positive) assessed by confocal laser scanning microscopy. (C) Distinct APs in FACS-purified PCMs. (D) Epifluorescence images and histochemical staining of atrial, atrioventricular (AV) nodal (acetylcholinesterase-positive/connexin43-negative), and ventricular regions in chimeric adult mouse heart (generated by blastocyst injection of αMHC-EGFP PSCs, A3 line). (E) Heart section from a chimeric mouse after injection of αMHC-EGFP PSCs (A3 line) into αMHC-nLacZ blastocysts. Light microscopy after X-gal staining (left panel); epifluorescence image of the same section showing PSC-derived EGFP-positive cardiomyocytes (middle panel); merged image did not show cells double-positive for EGFP and nLacZ, ruling out overt fusion events (right panel). Scale bars: (A) 10 μm, (B and D) 20 μm, (E) 50 μm.