J Clin Invest.
Mesodermal differentiation and cardiogenicity.
qPCR for pluripotency (Pou5f1), (cardio)mesoderm (T, Kdr, Isl1), and cardiac (Nkx2-5, Myh6) markers in undifferentiated (UD) cells and differentiating EBs in (A) PSC (n = 4–5 per group) and (B) ESC (n = 3–4 per group) cultures (data represent means ± SEM). Decrease in Nkx2-5 and Mhy6 in ESCs on culture day 7 + 6 was the consequence of nonmyocyte overgrowth. (C) Enumeration of beating EBs at the indicated culture days (n = 2–3 per time point; data represent means). (D) Quantity of EGFP-positive cardiomyocytes (CMs) per input stem cell (SC; 400 ESCs or PSCs per EB; cells from 5 single EBs were pooled for analytic FACS; n = 5–6) on culture day 5 + 10 (box plots indicate median with interquartile range; whiskers indicate minimal and maximal values). PSC line A3 and ESC lines ES4 and ES7 were generated from the same αMHC-EGFP mouse strain.