J Clin Invest.
Multilineage potential of PSCs in vitro and in vivo.
(A) RT-PCR analyses of lineage-specific transcripts in differentiating PSCs (PSC line B2). MEFs served as controls. Culture day 7; i.e., 2 days of hanging drop followed by 5 days in suspension culture. (+): Subsequent adhesion culture days. M, 100 bp DNA marker (full uncut gels are shown in the Supplemental Material). (B) EB culture day 22 (PSC line B3): pancytokeratin (pKRT), cytokeratin-18 (KRT18), α-fetoprotein (AFP), neurofilament protein M (NEFM), nebulin (NEB), GATA4. Respective lineage markers (red); nuclei (blue). Scale bars: 20 μm. (C and D) Comparison of teratoma size and composition 3 weeks after s.c. injection of ESCs and PSCs in SCID mice. Teratoma volume was quantified by ultrasonic biomicroscopy (representative image in C; data represent means ± SEM). H&E staining revealed multilineage potential of PSCs, including the capacity to generate cross-striated muscle (inset in D shows magnification of smaller boxed area). Scale bars: 50 μm.