Microchimerism of maternal origin persists into adult life
J. Clin. Invest. Sean Maloney, et al. 104:41 doi:10.1172/JCI6611 [
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Figure 2HLA-DRB5*01-specific PCR of DNA extracted from PBMCs of a scleroderma patient and a normal subject. The
DRB5 gene is present only on some HLA haplotypes. Both mothers had
DRB5*01 and both subjects did not. Lanes 3 and 4 are patient samples (different aliquots from the same draw date). Negative controls from homozygous cell lines with alleles similar to the patient’s are shown in lanes 5 and 6, and a positive homozygous control is shown in lane 7. Lanes 8–11 are 2 draw dates from a normal subject. Lanes 12 and 13 are negative cell line controls, and lane 14 is a positive homozygous cell line control. Lane 2 is blank. There was no alternative source of
DRB5*01 DNA in the normal subject. The scleroderma patient also had children with
DRB5*01 (see text).
