Min Luo, Xiaoqun Guan, Elizabeth D. Luczak, Di Lang, William Kutschke, Zhan Gao, Jinying Yang, Patric Glynn, Samuel Sossalla, Paari D. Swaminathan, Robert M. Weiss, Baoli Yang, Adam G. Rokita, Lars S. Maier, Igor R. Efimov, Thomas J. Hund, Mark E. Anderson
Langendorff-perfused hearts from WT mice treated with vehicle, STZ, and MitoTEMPO and STZ and AC3-I, MM-VV, and Ncf1–/– mice treated with STZ. (A) Heart rate. Overall P < 0.0001, ***P < 0.001 by 1-way ANOVA (n = 6–15 per group). (B) CSNRT. Overall P = 0.0003, **P < 0.01, *P < 0.05 by 1-way ANOVA (n = 5–8 per group). (C) Response to isoproterenol. *P < 0.0001 for 0 nM, **P = 0.0009 for 2 nM, ***P = 0.0001 for 20 nM, #P = 0.002 for 40 nM, ##P < 0.0001 for 100 nM (n = 5–8 per group). (D–F) Langendorff-perfused hearts isolated from STZ-treated mice after SAN painting with adenovirus expressing CaMKIIN and GFP or GFP alone. (D) Heart rates (*P = 0.01; n = 6–7 per group). (E) CSNRT (*P = 0.04; n = 6 per group). (F) Responses to isoproterenol (P > 0.05 for all doses excluding 100 nM, *P < 0.05; n = 6–7 per group).