J Clin Invest.
Intrinsic SND in diabetic mice.
Langendorff-perfused hearts from WT mice treated with vehicle, STZ, and MitoTEMPO and STZ and AC3-I, MM-VV, and Ncf1–/– mice treated with STZ. (A) Heart rate. Overall P < 0.0001, ***P < 0.001 by 1-way ANOVA (n = 6–15 per group). (B) CSNRT. Overall P = 0.0003, **P < 0.01, *P < 0.05 by 1-way ANOVA (n = 5–8 per group). (C) Response to isoproterenol. *P < 0.0001 for 0 nM, **P = 0.0009 for 2 nM, ***P = 0.0001 for 20 nM, #P = 0.002 for 40 nM, ##P < 0.0001 for 100 nM (n = 5–8 per group). (D–F) Langendorff-perfused hearts isolated from STZ-treated mice after SAN painting with adenovirus expressing CaMKIIN and GFP or GFP alone. (D) Heart rates (*P = 0.01; n = 6–7 per group). (E) CSNRT (*P = 0.04; n = 6 per group). (F) Responses to isoproterenol (P > 0.05 for all doses excluding 100 nM, *P < 0.05; n = 6–7 per group).