Monocytic AML in a Smap1–/– mouse (ID no. 831; see Table 3).
(A and B) Macroscopic and histological views of the liver and spleen. Note the remarkably enlarged liver and spleen in the Smap1–/– mouse. Sections of liver and spleen were stained by hematoxylin and eosin. Note the massive infiltration of leukemic cells in the Smap1–/– tissues. The leukemic cells were relatively small in size and possessed densely stained nuclei. Original magnification, ×40; ×100 (insets). Li, liver; Sp, spleen. (C) Peripheral blood (top) and bone marrow cell (bottom) smears. Scale bar: 10 μm. (D and E) Flow cytometry analyses of (D) bone marrow cells and (E) splenocytes prepared from Smap1+/+ and Smap1–/– mice. The cells were stained for hematopoietic lineage markers. The numbers indicate the percentage of cells in each gated box.