PP2A activation drives PTEN phosphorylation and nuclear localization via GSK3B.
(A) Representative immunofluorescence images of okadaic acid–treated (0.5 μM for 1 hour), FTY720-treated (10 μM for 6 hours) and SB216763-treated (5 μM for 12 hours) DU145 cells stained for PTEN (green). Scale bars: 50 μm. (B) Western blot analysis of DU145 cells treated with 5 nmol PP2A-A siRNA for 24 hours followed by 5 μM FTY720 for 12 hours as indicated. (C) Western blot analysis of DU145 cells treated with GSK3B siRNA for 24 hours followed by 5 μM FTY720 for 12 hours. All Western blots were quantified using ImageJ, and the values represent relative immunoreactivity of each protein normalized to respective loading control.