J Clin Invest.
SPRY2 loss–induced G1 arrest is mediated by nuclear accumulation of PTEN.
(A) Representative immunofluorescence (IF) images of indicated DU145 cells stained for PTEN (green), p21 (red), and DAPI (blue) (n = 3). Scale bars: 50 μm. (B and C) Representative immunofluorescence images of WT and Spry2+/– (B) MEFs and (C) prostate tissue stained for Pten (green). Red arrows indicate selected nuclei positive for Pten (n = 5). Scale bars: 100 μm. (D) Representative immunofluorescence images of indicated MEFs stained for Trp53 (green), p21 (red), and DAPI (blue). Scale bars: 100 μm. (E) Representative images and quantitation of DU145 orthotopic tumors stained for H&E, BrdU, and PTEN (*P < 0.01 **P < 0.001; n = 5, analyzed by Mann-Whitney test). Scale bars: 100 μm. Box and whisker plots show median (lines within boxes), interquartile range (bounds of boxes), and upper and lower range (whiskers). (F) SPRY2-LNCaP cells transfected with indicated plasmids were analyzed for cell-cycle profile after 24 hours (*P < 0.01; **P < 0.05 n = 3, analyzed by Mann-Whitney test). Data are presented as mean ± SEM.