SPRY2 loss–induced G1 arrest is mediated by nuclear accumulation of PTEN.
(A) Representative immunofluorescence (IF) images of indicated DU145 cells stained for PTEN (green), p21 (red), and DAPI (blue) (n = 3). Scale bars: 50 μm. (B and C) Representative immunofluorescence images of WT and Spry2+/– (B) MEFs and (C) prostate tissue stained for Pten (green). Red arrows indicate selected nuclei positive for Pten (n = 5). Scale bars: 100 μm. (D) Representative immunofluorescence images of indicated MEFs stained for Trp53 (green), p21 (red), and DAPI (blue). Scale bars: 100 μm. (E) Representative images and quantitation of DU145 orthotopic tumors stained for H&E, BrdU, and PTEN (*P < 0.01 **P < 0.001; n = 5, analyzed by Mann-Whitney test). Scale bars: 100 μm. Box and whisker plots show median (lines within boxes), interquartile range (bounds of boxes), and upper and lower range (whiskers). (F) SPRY2-LNCaP cells transfected with indicated plasmids were analyzed for cell-cycle profile after 24 hours (*P < 0.01; **P < 0.05 n = 3, analyzed by Mann-Whitney test). Data are presented as mean ± SEM.