SPRY2 KD decreases cell proliferation despite activation of mitogenic signaling.
(A) Whole cell lysates (WCL) of Nsi VC and SPRY2 KD DU145 clones were analyzed by Western blot (WB). (B and C) WCLs of (B) MEFs and (C) prostatic tissue from mice as indicated were analyzed by Western blot (n = 3). (D and E) BrdU incorporation analysis on (D) DU145 and (E) MEFs with indicated genetic alterations (*P < 0.001, **P < 0.05; n = 3; analyzed by Mann-Whitney test). Data are presented as mean ± SEM. (F) Representative IHC images and quantification for Ki67 staining in prostates of indicated mice. Scale bars: 100 μm. Arrows indicate Ki67-positive nuclei. (*P < 0.01, **P < 0.001; number of mice analyzed = 5 analyzed by Dunnett’s multiple comparison test). Box and whisker plots show median (lines within boxes), interquartile range (bounds of boxes), and upper and lower range (whiskers). All the Western blots were quantified using ImageJ, and the values represent relative immunoreactivity of each protein normalized to respective loading control.