J Clin Invest.
CT preserves insulin content and limits insulitis.
(A) Evaluation by ELISA of insulin content in pancreatic extracts from cured (14 weeks) and control experimental groups, as indicated. Data are expressed as ng/mg of pancreas. Shown are the individual values (symbols) overlaid with mean (horizontal line), box (25th and 75th percentile), and Tukey whiskers. Statistical significance between 2 groups was calculated using Mann-Whitney U test; *P < 0.05, **P < 0.01. (B) Pancreatic sections were stained for insulin (green), Ki-67 (red), and DNA by Hoechst (blue) and analyzed with confocal microscopy at ×63 original magnification. Representative Ki-67/insulin microphotographs documenting the absence of proliferating β cells in the pancreas are shown. (C) Pancreatic sections of the indicated groups were stained with H&E, and insulitis was scored as indicated.