ET-743 alone induces CEBPα, and ET-743 in conjunction with RSG induces PPARγ.
(A) MSCs from pTCp53null mice were treated with ET-743 or RSG in addition to ADM for 21 days. Drugs were changed with medium every 3 days. At the end of 21 days, cells were either stained with Oil Red O (to visualize lipid deposition) or analyzed via IHC for CEBPα and PPARγ expression. Original magnification, ×100 (Oil Red O); ×200 (IHC); ×1,000 (inset). (B) MSCs from pTCp53null and p53null mice were transfected with reporter assays for CEBPα and PPARγ after 21 days of differentiation as in A. Values denote average of 3 replicate experiments (see Methods).