Treatment with PGD2 antagonist BW A868C enhances rDC migration and T cell responses in IAV-infected mice.
(A) Twenty-two–month-old mice were inoculated i.n. with 50 μl 8 mM CFSE. Six hours after instillation, mice were infected with IAV together with BW A868C or vehicle. After 18 hours, single-cell suspensions were prepared from lung DLNs. The values represent the percentages of CFSE+ cells within the CD11c+MHCII+ DC population. Total CFSE+ DC numbers per LN are also shown. n = 4 mice for each age per experiment. Data are representative of 4 independent experiments. *P < 0.05. (B) Lung cells were harvested from 22-month-old B6 mice 8 days after IAV infection. Db/PA224 tetramer staining and total numbers of CD8 T cells and of Db/PA224 tetramer+ CD8 T cells are shown. Numbers represent the percentages of tetramer+ CD8 T cells. n = 4–6 mice/group/experiment. Data are representative of 5 independent experiments. *P < 0.05. (C) In vivo cytotoxicity assays were performed on day 7 p.i., and the percentage of killing was calculated, as described in Methods. n = 4–6 mice/group/experiment. Data are representative of 2 independent experiments. *P < 0.05.