Unimpeded but nondirectional CTL migration between anatomically nearby islets.
(A and B) During imaging, CTLs were often found to establish a 3D continuum between adjacent islets. A and B represent 3 adjacent islets (numbered islet 1, 2, and 3) captured in 2 individual Z-stacks over time. B corresponds to Supplemental Video 9. (C–E) 3D regions of interest were drawn in these inter-islet zones, and cellular motility was analyzed within these regions. D corresponds to Supplemental Video 10. Red lines show linear regression plot. Image dimensions: A, w/h = 1.52/d = 6/z = 22; B, w/h = 0.70/d = 6/z = 22; C, w/h = 1/d = 6/z = 22; D, w/h = 1.11/d = 6/z = 16; E, w/h = 1.51/d = 5/z = 28. Horizontal bars are means and associated error bars represent the SEM. Scale bars: 100 μm.