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Research Article

Glial cells in the mouse enteric nervous system can undergo neurogenesis in response to injury

Catia Laranjeira1, Katarina Sandgren1, Nicoletta Kessaris2, William Richardson2, Alexandre Potocnik3, Pieter Vanden Berghe4 and Vassilis Pachnis1

1Division of Molecular Neurobiology, Medical Research Council (MRC) National Institute for Medical Research (NIMR), London, United Kingdom.
2Wolfson Institute for Biomedical Research and Department of Cell and Developmental Biology, University College London, London, United Kingdom.
3Division of Molecular Immunology, MRC National Institute for Medical Research, United Kingdom.
4Laboratory of Enteric Neuroscience (LENS), TARGID, KULeuven, Leuven, Belgium.

Address correspondence to: Vassilis Pachnis, Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom. Phone: 0044.0.2088162113; Fax: 0044.0.2088162109; E-mail: vpachni@nimr.nerc.ac.uk.

First published August 25, 2011
Received for publication March 28, 2011, and accepted in revised form June 27, 2011.

The enteric nervous system (ENS) in mammals forms from neural crest cells during embryogenesis and early postnatal life. Nevertheless, multipotent progenitors of the ENS can be identified in the adult intestine using clonal cultures and in vivo transplantation assays. The identity of these neurogenic precursors in the adult gut and their relationship to the embryonic progenitors of the ENS are currently unknown. Using genetic fate mapping, we here demonstrate that mouse neural crest cells marked by SRY box–containing gene 10 (Sox10) generate the neuronal and glial lineages of enteric ganglia. Most neurons originated from progenitors residing in the gut during mid-gestation. Afterward, enteric neurogenesis was reduced, and it ceased between 1 and 3 months of postnatal life. Sox10-expressing cells present in the myenteric plexus of adult mice expressed glial markers, and we found no evidence that these cells participated in neurogenesis under steady-state conditions. However, they retained neurogenic potential, as they were capable of generating neurons with characteristics of enteric neurons in culture. Furthermore, enteric glia gave rise to neurons in vivo in response to chemical injury to the enteric ganglia. Our results indicate that despite the absence of constitutive neurogenesis in the adult gut, enteric glia maintain limited neurogenic potential, which can be activated by tissue dissociation or injury.

See the related Commentary beginning on page 3386.

Introduction

Establishment of functional circuits in the nervous system depends on the precise spatiotemporal generation of interconnected neuronal subtypes. In the CNS, postmitotic neurons are generated from neural stem cells (NSCs), which have the capacity to self-renew and differentiate into neuronal, astrocytic, and oligodendrocytic lineages (1). Over the last two decades, it has become clear that neurogenesis persists in the adult mammalian brain with astrocyte-like NSCs found in the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus (2). Although embryonic and adult NSCs share morphological and molecular properties, their exact relationship remains unclear. In vertebrates, the peripheral nervous system (PNS) is derived from neural crest stem cells (NCSCs), which migrate throughout the embryo and generate the majority of peripheral neurons and glia (3). Interestingly, the carotid body, an oxygen-sensing organ of the sympathoadrenal lineage, remains a neurogenic center throughout adulthood, with glia-like sustentacular cells giving rise to new neuron-like glomus cells in response to hypoxemia (4). Despite these studies, the presence and identity of stem cells in the ganglia of the PNS, their relationship to embryonic NCSCs, and their neurogenic potential in adult animals remain unknown.

The enteric nervous system (ENS), which regulates the peristaltic and secretory activity of the gut wall (5), is derived from enteric NCSCs (eNCSCs), which emerge during embryogenesis from the hindbrain and following stereotypical migratory patterns colonize the entire gastrointestinal tract (6). The transcription factor Sox10 is one of the earliest neural crest cell markers of ENS progenitors (7, 8). Sox10 is first detected in NCSCs that delaminate from the neural tube and migrate to invade the gut (9). Upon deletion of Sox10, enteric ganglia fail to form, demonstrating a critical role of this transcriptional regulator in ENS development (10). In vitro studies have suggested that Sox10-expressing undifferentiated progenitors in embryonic gut generate both enteric neurons (Sox10) and glia (Sox10+), but the neurogenic potential of these cells in vivo and its temporal regulation during gut organogenesis are currently unclear.

In rodents, enteric neurogenesis is restricted to embryonic stages and early postnatal life (11, 12). Nevertheless, self-renewing multipotent progenitors have been identified in cultures of post-neurogenic adult intestine (13, 14), while a recent report has provided evidence for enteric neurogenesis in adult animals in response to 5-hydroxytryptamine 4 (5-HT4) receptor activation (12). In addition, it has been suggested that inflammation or experimental intestinal obstruction can lead to a significant increase in the number of enteric neurons (15, 16). Although these observations suggest that neurogenic cells persist in enteric ganglia of adults, their identity and relationship to embryonic ENS precursors are currently unclear.

Here we combine genetic fate mapping, cultures of dissociated enteric ganglia, and an ENS injury model in mice to prospectively identify ENS progenitors and follow their developmental potential in vivo. Our studies demonstrate that Sox10-expressing enteric neural crest cells are highly neurogenic during embryogenesis, but neuronal differentiation is progressively reduced at late embryonic and early postnatal stages. Constitutive neurogenesis is undetectable in the ENS of adult animals, but remarkably, mature glial cells are capable of generating enteric neurons when placed in culture and in response to injury in vivo. Our data provide a framework for exploring the molecular mechanisms that control enteric neurogenesis in vivo and identify glial cells as a potential target for cell replacement therapies in diseases associated with congenital absence or acquired loss of enteric neurons.

Results

Sox10-expressing cells give rise to enteric neurons and glia in vivo. To examine the lineage relationship between Sox10-expressing neural crest cells and enteric neurons and glia, we fate mapped Sox10+ cells in vivo by combining a Sox10::Cre transgene (17) with the Cre-dependent R26ReYFP reporter (18). As expected, whole mount YFP immunostaining of E12.5 Sox10::Cre;R26ReYFP embryos showed expression of YFP in derivatives of neural crest cells, such as cranial sensory ganglia, dorsal root ganglia (DRG), sympathetic chain, and ENS (data not shown and Supplemental Figure 1; supplemental material available online with this article; doi: 10.1172/JCI58200DS1). The efficiency of Cre-mediated recombination in the ENS of Sox10::Cre;R26ReYFP mice was established by immunostaining short-term cultures of dissociated gut from E16.5 embryos for YFP and Sox10. YFP expression was detected in 82.7% ± 4.2% (391 cells, n = 3) of Sox10+ cells (Supplemental Figure 1), indicating that this double-transgenic system marks the majority of Sox10-expressing cells within the gastrointestinal tract.

To fate map Sox10-expressing cells in the ENS of Sox10::Cre;R26ReYFP embryos, we analyzed coexpression of YFP and appropriate lineage markers either in short-term cultures of dissociated gut from E16.5 embryos or on whole mount preparations of muscle strips with adherent myenteric plexus (MS-MP) from P30 animals. At E16.5, 27.8% ± 2.9% (523 cells, n = 3) of YFP+ cells expressed the glial marker brain fatty acid–binding protein (BFABP) (19), whereas nearly half of YFP+ cells expressed the pan-neuronal marker Tuj1 (47.1% ± 4.7%; 514 cells, n = 3) (Figure 1, A, B, and E). At this stage, approximately 25% of YFP+ cells were not labeled by either glial or neuronal markers, suggesting that they represented uncommitted progenitors. At P30, 64.3% ± 2.3% (1,981 cells, n = 3) of YFP+ cells coexpressed the glial marker S100β (19), while 34.4% ± 3.7% expressed the neuron-specific RNA binding protein HuC/D (2,180 cells, n = 3) (ref. 20 and Figure 1, C–E). In contrast to E16.5 embryos, only approximately 1% of YFP+ cells in the gut of P30 double-transgenic mice were negative for both neuronal and glial markers, suggesting that by this postnatal stage, most Sox10-expressing cells in the mouse gut have differentiated along the neuronal or glial lineage. Finally, no colocalization between YFP and the myofibroblast marker SMA (21) was detected in the gut of Sox10::Cre;R26ReYFP animals (644 cells, 3 E16.5 mice; 527 cells, 3 P30 mice). These findings demonstrate that enteric neurons and glia are derived in vivo from a common pool of Sox10-expressing progenitors.

Enteric neurons and glia are derived from a common pool of Sox10-expressingFigure 1

Enteric neurons and glia are derived from a common pool of Sox10-expressing progenitors. (A and B) Short-term cultures of dissociated gut from E16.5 Sox10::Cre;R26ReYFP transgenic embryos immunostained for YFP (green) and (in red) either the glial marker BFABP (A) or the neuronal marker TuJ1 (B). (C and D) Whole mount preparations of MS-MPs from P30 animals of the same genotype immunostained for YFP (green) and (in red) either the glial marker S100β (C) or the neuronal marker HuC/D (D). Arrows indicate double-positive cells. (E) Quantification of the fraction of YFP+ cells that belong to the neuronal of glial lineage of the ENS. Error bars indicate sem. Scale bars: 30 μm (A and B, insets of A and B), 60 μm (C and D, insets of C and D).

Stage-specific lineage labeling of Sox10-expressing ENS progenitors. To explore the dynamic changes in the developmental potential of ENS progenitors during pre- and postnatal stages, we generated transgenic mice expressing a tamoxifen-inducible form of Cre recombinase (iCreERT2) (22) under the control of Sox10 regulatory sequences (Sox10::iCreERT2 transgene). For this, a 170-kb phage artificial chromosome (PAC) containing 62 kb upstream and 57 kb downstream of the Sox10 gene (17) was modified by replacement of the coding region of Sox10 with iCreERT2 (ref. 23, Figure 2A, and Methods). Mice transgenic for the modified PAC were crossed to the R26ReYFP reporter strain to generate 4 Sox10::iCreERT2;R26ReYFP transgenic lines, called SER26, SER68, SER87, and SER93. The data presented in this report were obtained with the SER26 transgenic line but have been confirmed by analysis of additional lines.

Tamoxifen-dependent induction of YFP in Sox10::iCreERT2;R26ReYFP embryos reFigure 2

Tamoxifen-dependent induction of YFP in Sox10::iCreERT2;R26ReYFP embryos recapitulates Sox10 expression. (A) Targeting strategy for generating the Sox10::iCreERT2 transgene. Top: Schematic representation of the 170-kb Sox10 PAC. White and blue boxes represent noncoding and coding exons, respectively, and solid lines represent introns and flanking DNA sequences. The start of the coding sequence is shown (ATG). Relative positions of BglII and NotI restriction enzyme sites are shown. Bottom: Targeting cassette, which includes 5′ and 3′ homology regions (white boxes), the CreERT2 coding sequence (gray box), and the chloramphenicol resistance (Cmr) gene (green box) flanked by FRT sites (black arrows). Relative positions of AscI, BglII, SalI, SpeI, and PacI restriction sites and the location of the 3′ UTR probe used for Southern blot analysis of BglII-digested DNA are depicted. (B) Pregnant females were administered 4-OHT (0.2 mg/g) at E9.5, and Sox10::iCreERT2;R26ReYFP embryos were harvested and analyzed at E12.5. (CH) Serial sections from Sox10::iCreERT2;R26ReYFP embryos were hybridized with Sox10- or Cre-specific riboprobes (C, D, F, and G) or immunostained for YFP (E and H). Sections in CE represent DRG, while sections in FH represent the gut. (I and J) YFP immunostaining of whole mount preparations of gut from control (I) or tamoxifen-treated E12.5 embryos (J). Note the dramatic increase in the number of YFP-expressing cells in the gut of embryos exposed to tamoxifen. Oe, esophagus; St, stomach; Mg, midgut; Ce, cecum; Hg, hindgut. Scale bars: 50 μm (CH) and 600 μm (I and J).

To establish that the Sox10::iCreERT2 transgenes reflect the expression of the Sox10 locus, we performed in situ hybridization using Sox10- and Cre-specific riboprobes on adjacent sections of E12.5 SER26 embryos. Cre transcripts were restricted to structures expressing Sox10, such as DRG and the ENS (Figure 2, C, D, F, and G). Moreover, administration of 0.2 mg/g of 4-hydroxytamoxifen (4-OHT) at E9.5 resulted in YFP expression at these sites (Figure 2, B, E, and H). Indeed, in embryos and adult animals of all 4 transgenic lines, YFP was detected in established sites of Sox10 expression and in tissues derived from Sox10-expressing progenitors (Supplemental Figure 2 and data not shown).

To further examine whether the Sox10::iCreERT2 transgene can temporally activate the R26ReYFP reporter within the ENS, we analyzed YFP expression in the gut of E12.5 SER26 embryos treated with 4-OHT at E9.5. Relative to controls, the gut of 4-OHT–treated embryos contained a dramatically larger number of YFP-expressing cells (Figure 2, I and J). To quantify this effect, we immunostained dissociated gut cultures from control and 4-OHT–treated embryos for Sox10 and YFP. Upon administration of 4-OHT, 87.9% ± 8.6% of the Sox10+ cells of SER26 embryos (1,177 cells, n = 3) expressed the YFP reporter, but only 3.4 %± 1.1% (1,219 cells, n = 3) of the Sox10+ cells were YFP+ in control embryos (data not shown). These experiments, demonstrating a tamoxifen-dependent and cell type–specific activation of Cre recombinase in SER26 mice, establish a genetic system in which Sox10-expressing progenitors can be lineally marked at specific developmental stages in vivo.

The neurogenic potential of Sox10-expressing cells decreases after mid-gestation. Having developed a genetic system that marks Sox10+ cells at a given developmental stage, we next explored the temporal dynamics of neurogenesis by Sox10-expressing ENS progenitors at pre- and postnatal stages. To this end, SER26 animals were exposed to 4-OHT at different developmental stages, and the identity of lineally marked progeny within the gut was established by double immunostaining of adult MS-MP preparations for YFP and appropriate lineage markers. More specifically, tamoxifen was administered at E7.5, E8.5, E12.5, P0, P7, P30, and P84, and the population of enteric neurons generated from the lineally marked Sox10+ progenitors was examined at P84 (for 4-OHT administered between E7.5 and P30) or P140 (for 4-OHT administered at P84) (Figure 3A). In the absence of 4-OHT, only 0.6% ± 0.8% of HuC/D+ cells (1,589 cells, n = 3) were colabeled for YFP, consistent with low background levels of Cre activity (Figure 3, B and F). However, when 4-OHT was administered at E7.5, 23.5% ± 1.5% of the HuC/D+ neurons coexpressed YFP (1,280 cells, n = 3), indicating that approximately a quarter of enteric neurons found in the gut of P84 mice are generated from Sox10-expressing progenitors in E7.5–E8.0 embryos (Figure 3F). Sox10+ cells present in E8.5–E9.0 embryos (4-OHT injections at E8.5) generated 39.5% ± 10.6% (3,013 cells, n = 5) of enteric neurons in P84 animals (Figure 3, C and F). However, this fraction was progressively reduced at later stages: 36.9% ± 6.4% (1,606 cells, n = 4) of adult enteric neurons were derived from Sox10+ progenitors found in the gut of E12.5–E13.0 embryos, and only 7.8% ± 1% (2,748 cells, n = 4) of neurons were generated from P0 progenitors (Figure 3F). An even smaller fraction of enteric neurons was generated from Sox10+ progenitors that persist at postnatal stages: 2.8% ± 1.7% (2,471 cells, n = 3) were generated from P7 progenitors and 1.6% ± 1.1% (3,579 cells, n = 4) from P30 progenitors (Figure 3, D and F). Finally, animals treated with 4-OHT at P84 (0.6% ± 0.2%; 3,457 cells, n = 4) showed no YFP-labeled enteric neurons above background levels when analyzed at P140 (Figure 3, E and F), indicating that by this stage no neurons are generated by Sox10-expressing cells. Indeed, we found that all YFP-expressing cells in the myenteric plexus of animals exposed to 4-OHT at P84 represent S100β-expressing glial cells (3,081 cells, n = 4) (Figure 3, G and H). At this stage the efficiency of recombination of the fluorescent reporter was consistently greater than 50% (data not shown). For all the time points considered, the difference in the proportion of YFP-labeled cells in the HuC/D+ population between different regions of the small intestine was not statistically significant (data not shown). These experiments suggest that the neurogenic potential of Sox10+ progenitors in the mammalian gut is highest at mid-gestation but is subsequently reduced and becomes undetectable in adult animals.

Temporal changes in the neurogenic potential of Sox10-expressing eNCSCs.Figure 3

Temporal changes in the neurogenic potential of Sox10-expressing eNCSCs. (A) 4-OHT (0.2 mg/g) was administered to pregnant females or postnatal animals, and the gut of SER26 transgenic mice was analyzed at P84 (for injections at E7.5, E8.5, E12.5, P0, P7, and P30) or P140 (for injections at P84). (BE) Laser confocal microscopy images of representative whole mount MS-MP preparations from SER26 animals immunostained for YFP (green) and HuC/D (red) and counterstained with TOTO-3 (blue nuclear marker). MS-MP preparations were from P84 (BD) or P140 (E) mice that were either not treated (B) or treated with 4-OHT at the indicated time points (CE). Arrowheads indicate double-positive (YFP+HuC/D+) cells, which correspond to neurons generated after 4-OHT administration. Arrows indicate YFPHuC/D+ cells. (F) Quantification of the fraction of HuC/D+ neurons coexpressing YFP in the gut of P84 or P140 animals. Shown on the x axis are the time points of 4-OHT administration. (G) Laser confocal microscopy images of MS-MP preparations from P140 SER26 animals (tamoxifen administration at P84) immunostained for YFP (green), HuC/D (blue), and S100β (red). Insets are magnification of the boxed area showing YFP+HuC/DS100β+ cells (arrowheads). Arrows indicate YFPHuC/D+ neurons. (H) Quantification of the fraction of HuC/D+ and S100β+ cells within the YFP+ cell population. Error bars indicate sem. Scale bars: 60 μm (BE and G) and 10 μm (insets of BE and G).

YFP+ cells from the gut of adult SER26 animals acquire neurogenic properties in culture. Our failure to detect neurogenesis by Sox10+ cells in the gut of adult mice raises the question of the origin of the multipotential ENS progenitors isolated from cultures of adult gut (13, 14). The possibility that the source of these neurons is a small residual population of non-glial Sox10+ progenitors persisting within enteric ganglia after completion of enteric neurogenesis is not consistent with the finding that YFP was expressed exclusively in glial cells in our Sox10-driven reporter system (Figure 3, G and H). Therefore, we tested the hypothesis that mature enteric glial cells can exhibit neurogenic potential under certain conditions. For this, MS-MP preparations from SER26 adult mice (in which glial cells can be lineally marked) were dissociated 1 week after 4-OHT administration at P84 and cultured under conditions that promote the proliferation and self-renewal of ENS progenitors (8, 21, 24). Time course analysis of these cultures confirmed that within 12 hours after plating (day in vitro 1 [DIV1]), virtually all YFP+ cells coexpressed the glial marker glial fibrillary acidic protein (GFAP; 428 cells, n = 3), while the neuronal marker TuJ1 was present exclusively in YFP cells (Figure 4, A and E). At DIV4, YFP+ cells had increased in number and formed small colonies that were morphologically similar to the colonies generated by embryonic eNCSCs (refs. 8, 24, and Figure 4B). These colonies contained on average 9.6 ± 6.3 cells, the majority, of which (73.2% ± 11.3%) coexpressed the glial marker GFAP. However, 26.5% ± 10.9% of YFP+ cells expressed neither glial (GFAP) nor neuronal (Tuj1) markers (246 cells, n = 3), raising the possibility that they represent glial-derived eNCSCs (Figure 4, B and E). Consistent with this idea, most of the YFP+GFAP cells in DIV4 colonies were colabeled for Sox10, Phox2b (19, 25), and Sox2 (ref. 26 and Figure 4, F–I). In addition, a fraction of YFP+ cells in DIV4 colonies expressed Ascl1 (Mash1) (Figure 4I), a proneural gene that is expressed transiently in a subset of enteric neuron precursors (27). These findings suggest that cultured enteric glia are capable of generating multilineage ENS progenitors and activating neurogenic programs.

YFP+ glia from the ENS of adult SER26 mice generate neurons in culture.
   Figure 4

YFP+ glia from the ENS of adult SER26 mice generate neurons in culture. (AD) MS-MP cultures from 4-OHT–treated SER26 mice immunostained for YFP (green), TuJ1 (red), and GFAP (blue) at 12 hours (A), 4 days (B), 10 days (C), and 20 days (D) after plating. On DIV1, all YFP+ cells coexpressed GFAP (arrowhead in A), and all Tuj1+ neurons were YFP (arrow in A). On DIV4, the YFP+ population includes glial (YFP+Tuj1GFAP+) and non-glial non-neuronal (YFP+Tuj1GFAP) cells (arrowhead and arrow in B, respectively). After 10 DIV, YFP+ cells include glia (arrows in C), non-glial non-neuronal cells, and neurons (YFP+Tuj1+GFAP; arrowheads in C). On DIV20, most YFP+ cells are found in NLBs. (E) Quantification of the fraction of neurons and glia in the YFP+ population of DIV1, DIV4, and DIV10 cultures. Error bars indicate sem. (FI) DIV4 cultures immunostained for YFP and GFAP and Sox10 (F), GFAP and Sox2 (H), Phox2B (G), and Ascl1 (I). Sox10 and Sox2 were expressed by YFP+GFAP+ cells as well as by YFP+GFAP cells (arrows and arrowheads, respectively, in F and H). (JM) Immunostaining of DIV10 cultures for YFP and Tuj1 (J), nNOS (K), VIP (L), and NPY (M). (N) Immunostaining of 6-month-old cultures for YFP, HuC/D, and GFAP. In these cultures the YFP+ population included HuC/D+ neurons (arrow), GFAP+ glial cells (arrowhead), and HuC/DGFAP cells (diamond arrow). Scale bars: 60 μm (AD, N), 10 μm (FI), 10 μm (J), 10 μm (KM).

To further examine whether such putative progenitors are capable of generating mature enteric neurons, DIV7 adult gut cultures were shifted to differentiating conditions (laminin substrate and Neurobasal medium). Three days later (DIV10), we observed that 40.5% ± 3.8% of YFP+ cells coexpressed the pan-neuronal markers Tuj1 or HuC/D, 46.1% ± 0.6% expressed no lineage (neuronal or glial) marker, and 11% ± 3.3% coexpressed GFAP (265 cells, n = 3; Figure 4, C, E, and J). Interestingly, YFP+ neurons also expressed markers of enteric neuron subtypes, such as neuronal NOS (nNOS), vasoactive intestinal peptide (VIP), and neuropeptide Y (NPY), suggesting that YFP+ cells have the ability to generate neurons that express neurotransmitters found in the ENS (ref. 28 and Figure 4, K–M). As in dissociated embryonic gut (8), after several days in culture YFP+ cells started to pile up, and, by day 20, most were found within neurosphere-like bodies (NLBs), which eventually detached and floated in the medium (Figure 4D). YFP+ cells could be propagated without loss of expression of the reporter for at least 25 passages, and they were kept in culture for up to 6 months. Even in these late cultures, the YFP+ population included many cells that were YFP+HuC/DGFAP (Figure 4N), suggesting that they have the capacity to self renew.

Similar results were obtained using hGFAP::CreERT2;R26ReYFP mice (29), in which Cre was specifically activated in 19% ± 7.4% (988 cells, n = 5) of enteric glial cells (Supplemental Figure 3). Taken together, these studies indicate that although Sox10-expressing progenitors of the mammalian ENS lose their neurogenic capacity in vivo and differentiate into glial cells, under certain conditions they can reactivate their neurogenic potential and differentiate into mature enteric neurons.

Glial cells can generate functional synapse-forming neurons in culture. To investigate whether the glia-derived neurons displayed functional neuronal properties, we used Fluo-4 Ca2+ imaging to monitor neuronal activity in ENS cultures (30). To avoid the spectral overlap between the Ca2+ indicator and the YFP lineage reporter, we generated additional mouse lines that carry the Sox10::iCreERT2 transgene and the R26RFP635 allele, which is capable of expressing the red fluorescent protein FP635 (31) in a Cre-dependent manner (for the generation of the R26RFP635 allele, see Methods). ENS cultures were established from MS-MP strips of adult (>P84) tamoxifen-treated Sox10::iCreERT2;R26RFP635 transgenics, and patches of neuronal cells were identified morphologically using differential interference contrast (DIC) microscopy (Figure 5A). After Fluo-4 loading, all cells displayed dim green fluorescence (Figure 5C), while some of them expressed FP635, indicating that they derived from glial cells (Figure 5B).

Enteric glial cells generate functional neurons in vitro.Figure 5

Enteric glial cells generate functional neurons in vitro. (A) DIC image of enteric glial cell culture established from Sox10::iCreERT2;R26RFP635 mice. Numbers 1–4 indicate cells that are studied in detail. (B) Fluorescence image (recorded at 620/50 nm) of the cells shown in A. Note that cells 1 and 3 express the fluorescent protein FP635. (C) Fluo-4 signal (recorded at 525/50 nm) of the culture before stimulation. Note low background levels of signal in cells 1–4. (DG) AoT images in which only pixels responding to high K+ (D), EFS (E), and application of DMPP (F) and ATP (G) are shown. (H) Merge of images in B, D, and G. (I) Merge of images in B, E, and G. (J) Recordings of the responses of neurons 1 (red) and 2 (black) and glia 3 (red) and 4 (black) to high K+, EFS, DMPP and ATP. Neuron 1 and glia 3 express FP635. Insets show the fast upstroke in neuronal cells to high K+, compared with a slow secondary response in glial cells. Neurons typically show a fast response to high K+, EFS and DMPP, while glial cells show a slow response to high K+ and ATP. (K) Average response of FP635+ (red) and negative neurons to high K+. (L) Average response of FP635+ (red) and FP635 neurons to EFS. Scale bars: 20 μm.

A brief depolarization of cultured enteric neurons with high K+ (5 seconds, 75 mM) causes an immediate Ca2+ influx, resulting in a sharp increase in fluorescence that reaches maximum values in less than 2 seconds before decaying exponentially (30). The fast Ca2+ response to K+ depolarization is a characteristic feature of enteric neurons that distinguishes them from glia, which show a delayed response (7 seconds required to reach the maximum) (32). Enteric neurons also show a robust response to electrical field stimulation (EFS, 20 Hz, 2 seconds, 20 mA) and the nicotinic agonist dimethylphenylpiperazinium (DMPP; 10–5 M) (32). Application of ATP (10–5 M) on the other hand can be used to confirm glial cell identity (32). Using these stimuli, a neuronal Ca2+ signaling fingerprint could be determined, which is defined by a strong and fast response to high K+, EFS, and DMPP and no or delayed response to ATP. For enteric glia, the opposite is true. Figure 5 shows the responses of 4 cells (numbered 1–4), which, based on the expression of FP635 and morphological characteristics, were identified as: cell 1, FP635+ neuron; cell 2, FP635 neuron; cell 3, FP635+ glia; cell 4, FP635 glia. Images of these cells following exposure to the various stimuli are shown in Figure 5D (high K+), Figure 5E (EFS), Figure 5F (DMPP), and Figure 5G (ATP). The time course of Ca2+ responses for the 4 cells is shown in Figure 5J. In total we have analyzed a group of 74 cells, 34 of which were positively identified as neurons (14 FP635+ and 20 FP635) and 40 as glia (17 FP635+ and 23 FP635). The characteristic Ca2+ signaling fingerprint was present in the majority of FP635+ neurons. Challenged with high K+, all neurons displayed the typical fast response, which did not differ in amplitude or duration between control and FP635-expressing neurons (Figure 5K). EFS elicited Ca2+ transients in 75% of analyzed neurons (24 of 34, n = 3), which was not different between FP635+ and FP635 cells (65%, 9 of 14, P = 0.4, χ2 test). Also, the amplitude of the response in the two groups was similar (Figure 5L). DMPP elicited clear responses in 80% (26 of 34) of the neurons, and although the DMPP response amplitude did not differ, the proportion of responders was somewhat lower in the FP635-expressing population (57%, P = 0.02, χ2 test). Results of independent Ca2+ imaging experiments with cultures established from SER26 mice were consistent with the data obtained from Sox10::iCreERT2;R26RFP635 animals (data not shown).

Enteric neurons in culture establish functional networks with synaptic release sites (33). To investigate whether enteric glia–derived neurons were equally capable of integrating into the neuronal network and establishing functional synaptic connections, we employed immunohistochemical and Ca2+ imaging techniques. More specifically, we analyzed cultures from adult tamoxifen-treated SER26 mice to identify sites of neurotransmitter release using the general synaptic vesicle marker synaptophysin (33). Triple labeling for synaptophysin, nNOS, and YFP (indicating glia origin) revealed the presence of synaptic vesicles (Figure 6, A–D) in YFP+ neurons. Ca2+ imaging was used to monitor presynaptic activity in individual varicosities and putative release sites in culture (34). After loading with Fluo-4, characteristic neuronal Ca2+ fingerprints could be recorded from these sites, which showed a fast fluorescent upstroke to depolarization (high K+) and to the nicotinic agonist DMPP (Figure 6, G–I). All cells, including neurons, shown in Figure 6, E–J, were YFP+, and thus of glial origin. Together, these findings suggest that enteric glia cells from adult animals are capable of generating functional enteric neurons in culture.

Glial cells generate synapse-forming neurons in vitro.Figure 6

Glial cells generate synapse-forming neurons in vitro. (AD) Fluorescence images of the same field of view of an ENS culture established from adult (>P84) 4-OHT–treated SER26 animals. Culture was immunostained for YFP (A), nNOS (B), and synaptophysin (SYN; C). A merge of images in AC is shown in D. Arrows indicate a YFP+nNOS+ neuron that exhibits punctuate expression of the synaptic protein synaptophysin. (E) DIC image of an ENS culture established from Sox10::iCreERT2;R26RFP635 mice. Arrows point to varicosities analyzed in detail in the other panels. (F) Fluo-4 image without stimulation. (G) AoT at the varicosities indicated in E upon high-K+ stimulation. (H) Typical Ca2+ transients in the indicated neuronal varicosities following high-K+ (red) and DMPP (blue) stimulation. (I) AoT of DMPP stimulation. (J) Merge of images in F, G, and I in which the varicose nerve fiber is visible in purple. Scale bars: 20 μm.

YFP+ glial cells generate neurons in vivo after injury. Next we examined whether enteric glia exhibit neurogenic potential in vivo. Since we could not detect neurogenesis in the ENS of adult (>P84) SER26 transgenic mice (Figure 3), we hypothesized that the neurogenic potential of enteric glial cells may be revealed only upon disruption of tissue homeostasis. To test this premise, we exploited the Sox10::iCreERT2;R26ReYFP lineage marking system in combination with the cationic detergent benzalkonium chloride (BAC), which ablates locally the myenteric plexus in a dose-dependent manner when applied to the serosal surface of the intestine (35). BAC was applied to a small area of the small intestine of adult SER26 mice (>P84) that had been previously treated with tamoxifen to lineally mark glial cells. MS-MP preparations from the treated and adjacent areas were analyzed at different times after BAC application for expression of YFP, the pan-neuronal marker HuC/D, and the glial marker S100β. Consistent with previous reports (36, 37), 3 days after treatment, enteric ganglia were eliminated from the region of BAC application (Figure 7, A and B). One month later the treated area was colonized by glial cells and was invaded by neuronal projections. No YFP+ neurons were found in the aganglionic intestinal segment or in the area adjacent to the BAC-treated region, where most ganglia included YFP+ glial cells but no neurons, as assessed by expression of HuC/D (n = 3). Away from the treated area, we found morphologically normal ganglia containing YFPHuC/D+ neurons and YFP+S100β+ glial cells (Figure 7, C–E). However, when analyzed at approximately 3 months (70–90 days) after BAC treatment, enteric ganglia bordering the aganglionic area contained a relatively large fraction of YFP+HuC/D+ cells (9.0% ± 4.0%; 3,197 HuC/D+ cells, n = 4), indicating that they derived from YFP-marked glial cells. The percentage of YFP+ enteric neurons detected within enteric ganglia was inversely proportional to their distance from the treated area, ranging from 0.5% (background level at segments distant from treated region) to 9% (adjacent to the treated area) (F[2] = 5.613; P = 0.03; ANOVA; Figure 7, F–I). In control mice, the frequency of YFP+HuC/D+ neurons throughout the gut was within the expected background levels and comparable to that observed in distally located gut regions of BAC-treated animals. Together, these experiments suggest that enteric glial cells have neurogenic potential in vivo and are capable of generating enteric neurons in response to ENS injury.

YFP+ glial cells from the ENS of adult SER26 mice can generate neurons in vFigure 7

YFP+ glial cells from the ENS of adult SER26 mice can generate neurons in vivo. (A) Laser confocal microscopy of MS-MP preparations from the gut of 4-OHT– and BAC-treated SER26 mice (P84 or older). Inset shows absence of YFP+, HuC/D+, or S100β+ cells in the treated area. (B) Schematic representation of enteric plexus that includes an area that as a result of BAC treatment becomes aganglionic (area 3). (CH) Laser confocal microscope images of MS-MP preparations representing areas of the enteric plexus that are located distant to (area 1; C and F), adjacent to (area 2; D and G), or within the BAC-treated area of the gut (area 3; E and H) and analyzed 1 month (CE) or 3 months (FH) after BAC treatment. MS-MP strips were immunostained for YFP (green), HuC/D (red), and S100β (blue). Arrowheads in insets of G indicate YFP+HuC/D+ double-labeled cell. Arrows indicate YFP+HuC/DS100β+ cells. Diamond arrow in D indicates space within the ganglia suggesting that neuron loss has occurred. (I) Quantification of the fraction of YFP+HuC/D+ cells in areas 1, 2, and 3. Note that the fraction of YFP+ neurons present within enteric ganglia of area 2 is much greater relative to that in area 1. *F(2) = 5.613; P = 0.03; ANOVA. Error bars indicate sem. Scale bars: 600 μm (A), 100 μm (C, E, F, H, and inset of A), 60 μm (D and G), 30 μm (insets of E and H), 10 μm (insets of C, D, and F), 10 μm (insets of G).

Discussion

Identification and characterization of eNCSCs in vivo are crucial for understanding how their orchestrated self-renewal and differentiation generate functional neuronal networks within the gut. So far, the properties of eNCSCs have been deduced from cell culture and transplantation studies of cells isolated from the gut on the basis of molecular and functional characteristics (8, 13, 38). Using genetic fate mapping, we demonstrate here that the Sox10-expressing neuroectodermal derivatives of the mammalian gut generate both lineages of the mammalian ENS and therefore are likely to represent the eNCSCs in vivo.

The majority of neurons in the adult ENS are derived from Sox10-expressing progenitors that reside in the gut of mid-gestation mouse embryos. As development proceeds, the fraction of enteric neurons generated from increasingly older pools of progenitors is gradually reduced and eventually, at some stage between P30 and P84, no new neurons are generated from the available Sox10+ cells. From that point onward, our Sox10-driven reporter system exclusively labels glial cells, as identified by the expression of enteric glia markers, such as GFAP and S100β. The total number of Sox10-expressing cells within the gut does not decrease over time, but the gradual reduction of neurogenesis is coupled to the increasing representation of gliogenic precursors and glial cells, which also express Sox10 (19, 39). The neurogenic to gliogenic switch of Sox10+ progenitors could result from changes in the gut microenvironment, or the gradual accumulation of neuron-derived signals that reach levels sufficient to induce gliogenic fates. Alternatively, the observed changes in neurogenic potential may reflect changes in the intrinsic properties of ENS progenitors, which cease to respond to neuron-promoting signals. In support of this view, Kruger et al. have shown that in comparison to embryonic eNCSCs, their postnatal counterparts exhibit decreased sensitivity to the neurogenic effect of BMP4 and increased sensitivity to the gliogenic activity of the Notch ligand Delta-Fc and neuregulin (13).

A similar dynamic profile of neurogenic (high early, low later) and gliogenic (low early, high later) potential of precursors has been observed in other parts of the nervous system, including the cortex. However, in contrast to the CNS, where the generation of neurons and glia are temporally segregated (40), we observed considerable overlap between the periods of enteric neurogenesis and gliogenesis. The first glial precursors appear in the gut at approximately E11.5 (19), but eNCSCs generate new neurons throughout embryogenesis and beyond P30 (ref. 11 and this study). These findings strengthen the idea that new neurons continue to be integrated into the ENS circuitry long after functional neuronal networks have been established in the gut.

The failure of Sox10+ cells in the gut of P84 animals to generate enteric neurons is in agreement with an extensive series of BrdU incorporation studies described in a companion to the present article, by Joseph et al. (41), and earlier reports that have been unable to identify newly born neurons in the intact adult mammalian ENS (11, 12). Therefore, unlike the CNS, which harbors NSCs and sustains neurogenesis throughout life, the ENS of adult animals does not support constitutive neurogenesis. Nevertheless, self-renewing multilineage progenitors of the ENS have been identified in adult intestine (13). Moreover, administration of 5-HT4 agonists induced enteric neurogenesis in adult mice (12). Our present studies suggest that the multilineage progenitors of adult ENS are enteric glial cells that under certain in vitro and in vivo conditions (such as injury) can activate their neurogenic potential and generate enteric neurons. It is currently unclear whether all glial cells in the adult intestine can, under certain conditions, serve as neurogenic precursors and whether neurogenic potential is restricted to a small fraction of cells expressing glial markers.

Once thought to be passive and merely supporting partners of neurons, glial cells have recently emerged as active regulators of many neuronal functions (42). Furthermore, several reports have highlighted the close relationship between NSCs and glia. Ongoing neurogenesis in the CNS is ensured by GFAP-expressing progenitors with morphological and molecular characteristics of astrocytes (43), and glial cells are the source of new neurons in the injured retina (44) and adult carotid body (4). Our findings suggest that in addition to their regulatory and supportive roles, enteric glia may participate, when and if necessary, in neurogenesis. Although the neurogenic potential of enteric glia is likely to remain dormant under the constraints of the adult ENS niche, such potential can be activated when their microenvironment is altered, either by elimination of the niche (e.g., dissociation and culture) or injury of enteric ganglia.

In culture, neuronal differentiation is preceded by extensive proliferation and de-differentiation of glial cells into a cell type that has molecular and morphological characteristics of multilineage ENS progenitors. It is currently unclear whether proliferation and de-differentiation are critical for activating the neurogenic potential of enteric glia in vivo. Interestingly, a recent report that describes differentiation of cortical astroglia into neurons provides evidence that cell division is not a prerequisite for neuronal differentiation, thus arguing for a direct fate conversion (45). Moreover, we have so far failed to identify non-neuronal non-glial YFP+ cells in the vicinity of the BAC-treated area, as has been observed in culture. We recognize of course that it is currently impractical to follow systematically the dynamics of marker expression by glial cells in response to injury in vivo, and it is possible that such cells are generated in response to BAC treatment but have simply escaped our detection. With these caveats in mind, we suggest that although the initial trigger that activates neurogenesis from glial cells both in vitro and in vivo is similar, the neurogenic script followed subsequently by the responding cells in the two experimental paradigms may be different.

This idea is further supported by our studies using hGFAP::CreERT2;R26ReYFP transgenic mice, in which tamoxifen administration labels approximately 20% of enteric glia in adult animals. Although in culture we observed robust activation of the neurogenic potential of adult enteric glia marked by this transgene, we were unable to detect YFP+HuC/D+ enteric neurons using the chemical injury paradigm employed for the Sox10::CreERT2;R26ReYFP mice (Supplemental Figure 3 and data not shown). These observations are consistent with the findings of Joseph et al. (41) and raise the interesting possibility that the neurogenic glia in the gut of adult mice represent a subset of Sox10-expressing cells that are not marked by the GFAP::Cre transgenes used in the two studies. It is possible that in culture under the influence of strong neurogenic and proliferative signals, most enteric glia can differentiate into neurons, while in vivo neurogenic potential can be activated only in a subset of Sox10-expressing glial cells. Further studies will be necessary to characterize the properties of the putative neurogenic glia in the ENS of adult mice.

What is the nature of the signals that trigger the neurogenic potential of glial cells? We suggest that both in culture and in vivo, the initiating event is related to the disruption of the intercellular interactions that have been established within the tightly packed enteric ganglia and are required to maintain glial cells in a “dormant” state. Such interactions are clearly lost upon dissociation and culture of enteric ganglia. In addition, the cellular partners of glial cells within enteric ganglia may also change in response to BAC treatment, as suggested by our observation that ganglia next to the treated areas appear to have lost many HuC/D+ neurons. Such neuronal loss may be secondary to the elimination of postsynaptic targets in the aganglionic region induced by BAC treatment. Irrespective of the mechanisms, the elimination of neurons in otherwise healthy ganglia is likely to change the “niche” of glial cells, which by “sensing” the absence of neurons in their vicinity can activate their erstwhile neurogenic program.

Although the majority of enteric glia are found within enteric ganglia, our findings do not exclude the possibility that neurogenic precursors in the adult ENS are extraganglionic. Indeed, Liu and colleagues have previously suggested that Sox10-expressing cells located outside the ganglia are capable of generating enteric neurons in response to 5-HT4 receptor activation (12). The relationship between the gliogenic precursors identified in our study and the Sox10+ precursors reported by Liu et al. warrants further investigation. Also, as serotonin is likely to be present in enteric ganglia, it would be interesting to examine whether this factor is sufficient to drive the differentiation of enteric glial cells into neurons in culture and in vivo. Irrespective of the exact source of precursors and the molecular mechanisms that activate their neurogenic potential, our studies indicate that the enteric neurons derived in culture from glial precursors are functional. This conclusion is based on the analysis of intracellular Ca2+ responses (to stimuli such as K+ depolarization and pharmacological and electrical stimulation) of glia-derived neurons and their putative synaptic contacts.

Neuronal degeneration is a common feature in a number of gastrointestinal disorders, such as inflammatory bowel disease (IBD) and post-enteritis irritable bowel syndrome (46, 47). The potential link between inflammation and neuronal degeneration was first suggested by early histopathological studies showing that, in the context of inflammation, neuronal degeneration is often accompanied by other abnormalities, including hypertrophy of ganglia and nerve bundles and/or hyperplasia of glial cells (16). However, Joseph et al. (41) were unable to detect neurogenesis by BrdU incorporation in the gut of mice that were inflamed by chemical treatment or bacterial infection. Nonetheless, if neurogenesis occurs without cell division in response to inflammation, perhaps by direct differentiation of enteric glia into neurons, this could have therapeutic implications for the treatment of severe inflammatory conditions of the gut. Understanding the mechanisms that regulate the neurogenic potential of enteric glia could facilitate the design of novel therapeutic strategies for the treatment of conditions associated with congenital absence of enteric neurons, such as Hirschsprung disease.

Methods

Animals. Generation of the Sox10::Cre and hGFAP::CreERT2 transgenic mice and the R26ReYFP reporter strain have been described previously (17, 18, 29). Sox10::iCreERT2 transgenic mice were generated by pronuclear injection of a modified PAC expressing a tamoxifen-inducible Cre recombinase (iCreERT2) (48) under the control of Sox10 regulatory sequences. The original PAC (RP21-529-I6 from RPCI mouse PAC library 21, UK HGMP Resource Centre; ref. 17) was modified by homologous recombination in bacteria using a targeting vector containing 5′ (AscI-SalI 460-bp) and 3′ (SpeI-PacI 440-bp) homology regions, an iCreERT2 cassette (3.3 kb) (22), and the chloramphenicol resistance gene (CmR), which was flanked by FRT sites and therefore could be removed by transient activation of Flp recombinase in bacteria (Figure 2A and ref. 23). Separation of Sox10iCreERT2 sequences from the vector and generation of transgenic animals were carried out as described previously (49).

To generate the R26RFP635 reporter strain, the coding sequences of the red fluorescent protein FP635 (31) was inserted into the pROSA26 targeting vector downstream of a loxP-flanked neomycin resistance cassette containing three transcriptional stop signals (18). This plasmid was subsequently linearized with KpnI and used for electroporation of R1 ES cells. ES cell clones with a correctly targeted R26R allele were identified by Southern blotting and used to establish the R26RFP635 reporter mouse strain.

Timed matings for embryonic studies were generated by crossing double-transgenic animals with Parkes (outbred) mice. Noon of the day of vaginal plug detection was considered to be E0.5.

4-OHT (Sigma-Aldrich) was dissolved in an ethanol/sunflower oil (1:9) mixture at 10 mg/ml and stored at 4°C (maximum 2 weeks) or at –20°C (for months). For detailed characterization of Cre activity, 0.2 mg/g of 4-OHT was administered by intraperitoneal injection to pregnant Parkes females crossed with Sox10::iCreERT2;R26ReYFP males 9.5 days post coitum, and the embryos were analyzed at E12.5. To analyze neurogenesis in postnatal and adult ENS, we injected Sox10::iCreERT2;R26ReYFP or hGFAP::CreERT2;R26ReYFP animals intraperitoneally with 0.1–0.2 mg/g of 4-OHT for 2 consecutive days. Control mice were not injected or injected with an ethanol/sunflower oil (1:9) mixture.

Genotyping was carried out by PCR using the following primer pairs: Cre, 5′-ATCCGAAAAGAAAACGTTGA-3′, 5′-ATCCAGGTTACGGATATAGT-3′; iCreERT2, 5′-ATCCGAAAAGAAAACGTTGA-3′, 5′-ATCCAGGTTACGGATATAGT-3′; R26ReYFP, 5′-GCTCTGAGTTGTTATCAGTAAGG-3′, 5′-GCGAAGAGTTTGTCCTCAACC-3′, 5′-GGAGCGGGAGAAATGGATAGT-3′. All animal experiments were approved by the local National Institute for Medical Research ethical review panel in compliance with the United Kingdom Animal (Scientific Procedures) Act of 1986.

Cell culture. For short-term culture of dissociated embryonic intestine, whole gut from E16.5 embryos was dissected in L15 medium (Invitrogen), washed with Ca2+- and Mg2+-free PBS (Invitrogen), and digested for 3 minutes with 1 mg/ml dispase/collagenase (Roche) at room temperature (RT). Dissociated tissue was washed with PBS and plated onto fibronectin-coated (20 μg/ml; Sigma-Aldrich) SONIC-SEAL slide wells (VWR) in OptiMEM (Life Technologies) supplemented with l-glutamine (1 mM, Life Technologies) and penicillin/streptomycin antibiotic mixture (Life Technologies). Cultures were maintained for up to 5 hours in an atmosphere of 5% CO2.

To culture enteric crest–derived cells from adult mice, MS-MPs were isolated from the small intestine, washed in 0.1% penicillin/streptomycin in PBS, cut into small pieces, and then incubated in 1 mg/ml collagenase (Sigma-Aldrich), 100 μg/ml DNase I (Sigma-Aldrich) for 1 hour at 37°C. After 2 washes in PBS, dissociated tissue was washed in NCSC culture medium, containing 15% chicken embryo extract, 20 ng/ml FGF (R&D Systems), and 20 ng/ml EGF (Merck Biosciences) (21). Cells were plated onto fibronectin-coated (20 μg/ml; Sigma-Aldrich) LabTek 8-well slide chambers (VWR) in NCSC medium and maintained in an atmosphere of 5% CO2 for up to 6 months. Neuronal cultures were established by plating cells at medium density onto poly-d-lysin– and laminin-coated LabTek 8-well slide chambers in Neurobasal medium (Invitrogen) supplemented with 1% N2 (Invitrogen) and 2% B27 (Invitrogen).

Immunofluorescence and in situ hybridization. Immunofluorescence of dissociated gut cultures was carried out as reported previously (8). MS-MP preparations were fixed in 4% (w/v) PFA in PBS for 30 minutes at RT, whereas embryos and guts were fixed for 2 hours at 4°C. For sectioning, fixed tissue was cryoprotected overnight in 30% sucrose in PBS, incubated in PBS containing 15% sucrose and 7.5% gelatin, and then frozen in Tissue-Tek OCT on dry ice. Cryosections were cut at 12 μm. The antibodies used for immunofluorescence were: rabbit anti-BFABP (1:1,000, Millipore), rabbit anti-GFAP (1:300, Dako), mouse anti-HuC/D (1:500, Molecular Probes, Life Technologies), mouse anti-Ascl1 (1:500, a gift from F. Guillemot, NIMR, London, United Kingdom), rabbit anti-nNOS (1:200, Zymed, Invitrogen), rabbit anti-NPY (1:50, Biogenesis), rabbit anti-Phox2b (1:200, a gift from C. Goridis, Ecole Normale Supérieure, Paris, France), rabbit anti-SMA (1:250, Sigma-Aldrich), goat anti-Sox10 (1:200, Santa Cruz Biotechnology Inc.), rabbit anti-S100β (1:500, DAKO), mouse anti-synaptophysin (1:200, Dako). Rabbit anti-GFP (1:1,000, Molecular Probes, Life Technologies) and rat anti-GFP (1:1,000, Molecular Probes, Life Technologies) antibodies were used to study expression of the YFP reporter, and the two terms are used interchangeably. Primary antibodies were applied overnight at 4°C. Secondary antibodies were Alexa Fluor 488–, Alexa Fluor 568–, or Cy5-conjugated anti-rat, -rabbit, -mouse, or -goat (1:500, Molecular Probes, Life Technologies) and were applied for 2 hours at RT. All antibodies were diluted in PBS containing 0.1% Triton-X100 and 10% sheep serum or in PBS containing 0.1% Triton X-100, 1% bovine serum albumin, and 0.15% glycine. TOTO-3 iodide (Molecular Probes, Life Technologies) was used to detect the cell nuclei. Following antibody treatment, samples were mounted in VECTASHIELD mounting medium containing DAPI (Vector Laboratories).

In situ hybridization histochemistry on cryosections was carried out as described previously (50). The following DNA fragments were used to generate digoxigenin-labeled riboprobes: rat Sox10, an EcoRI-BamHI 800-bp fragment (a gift from M. Wegner, Institut für Biochemie, Erlangen, Germany), and iCre, a 900-bp fragment spanning the entire iCre ORF (51).

[Ca2+]i –Fluo-4 imaging. Progenitor cells derived from MS-MP preparations isolated from Sox10::iCreERT2;R26RFP635 or Sox10::iCreERT2;R26ReYFP animals were cultured for 7–10 days in NCSC medium and then seeded onto laminin/poly-d-lysine–coated glass coverslips in Neurobasal medium. After 5 days, patches of neurons could be identified by DIC microscopy. Cells were loaded with 5 μM Fluo-4 AM (20 minutes), washed, and transferred to a cover glass chamber mounted on the microscope stage. The cells were constantly perfused with control or drug containing HEPES-buffered solution that was gravity fed (1 ml/min) via a multi-barrel perfusion system. The HEPES-buffered Krebs solution contained in mM: 148 NaCl, 5 KCl, 2 CaCl2, 1 MgCl2, 10 glucose, 10 HEPES (pH, 7.38). The high-K+ buffer used for neuron identification contained 75 mM KCl, and Na+ was reduced to 78 mM. For EFS (1 second, 20 Hz, 40 mA, 1-ms pulses, WPI A385 stimulator) coverslips containing cultured cells were transferred to a recording chamber with two parallel Pt wires. All recordings were made at RT.

Fluorescence images were recorded using an inverted Zeiss Axiovert 200M microscope equipped with a TILL Poly V light source (TILL Photonics) and cooled CCD camera (PCO Sensicam-QE) using TillVisION (TILL Photonics). TurboFP635 (Evrogen) and YFP were excited, respectively, at 560 nm and 475 nm. Fluorescence images were collected at 620/50 nm and 525/50 nm for FP635 and YFP, respectively. Changes in intracellular Ca2+ concentration ([Ca2+]i) reflected in Fluo-4 fluorescence intensity were recorded at 525/50 nm. All image analysis was performed with custom-written routines in IGOR Pro (Wavemetrics). Regions of interest (ROIs) were drawn over individual cells, fluorescence intensity was normalized to the basal fluorescence at the onset of the recording for each ROI, and peaks were analyzed. A peak was considered if the signal rose above a cutoff, which was set to the baseline plus 5 times the intrinsic noise level and the maximum [Ca2+]i peak amplitude was determined. Color-coded images of responding cells (activity over time [AoT]) were generated by an automated routine that attributes, on a pixel by pixel basis, the maximum value of that pixel in a certain time window. The value was only assigned if a maximum significantly different from the starting value was observed; otherwise, the pixel in the AoT image was set to 0. Thereby, all fluorescence information that was not changing in a given time window was filtered out. These images were falsely colored (green or blue) and overlaid with other AoT images in order to make differences in responses of neurons and glial cells visible.

BAC treatment. Sox10::iCreERT2;R26ReYFP double transgenics, P84 or older, were injected intraperitoneally with 0.1–0.2 mg/g of 4-OHT for 2 consecutive days. All animals were operated upon general isoflurane anesthesia. A laparotomy with a midline incision was performed, and a portion of the small intestine was exposed. Paper gauze soaked in 0.1% BAC (Sigma-Aldrich) in 0.9% Krebs solution was wrapped around 1 cm of the exteriorized intestine delineated with two serosal suture tags. The treatment was carried out for 5 minutes, after which the paper gauze was removed and the treated area and the peritoneum were thoroughly flushed with Krebs solution. Animals were sacrificed 3, 30, or 80 days after treatment. The treated segment of bowel and untreated segments orad and aborad to the treated area were harvested from each animal, and MS-MPs were isolated for immunofluorescence.

Image processing and cell counting. YFP expression in Sox10::Cre;R26ReYFP and Sox10::iCreERT2;R26ReYFP double-transgenic embryos and guts was analyzed using a Zeiss M2-Bio stereofluorescence microscope. Images were acquired with a Hamamatsu ORCA ER Digital Camera and Openlab software (Improvision). Immunostained cultures and MS-MP preparations were examined with a Zeiss epifluorescence Axioplan and Openlab software or with a Bio-Rad/Radiance 2100 confocal laser scanning microscope and LaserSharp software (Bio-Rad), using standard excitation and emission filters for visualizing DAPI, Alexa Fluor 488, Alexa Fluor 568, and Alexa Fluor 647. All images were processed with Adobe Photoshop CS 8.0 (Adobe Systems).

Statistics. Data are presented as mean ± SEM, with the number of experiments shown in parentheses. Cell counting data were subjected to ANOVA or χ2 test for statistical significance. Differences were considered statistically significant when the P value was less than 0.05.

Supplemental data

View Supplemental data

Acknowledgments

We thank François Guillemot (NIMR) for the anti-Ascl1 antibody and Christo Goridis (Ecole Normale Supérieure, Paris) for the anti-Phox2B antibody. We are very grateful to members of our laboratories, in particular Dipa Natarajan and Matthew Grist, for technical suggestions. We thank Biological Services (NIMR) and Mauro Tolaini for helping with the generation of transgenic mice. We thank Sean Morrison for useful comments on the manuscript and for sharing unpublished information with us. Work in V. Pachnis’s laboratory is supported by the MRC. C. Laranjeira was supported by a predoctoral fellowship (SFRH/BD/22297/2005) from Fundação para a Ciência e a Technologia (FCT) and the MRC. K. Sandgren was supported by postdoctoral fellowships from the Wenner Gren Foundation, Wera Ekström Foundation, and the Swedish Society for Medical Research, with supporting grants from the H.R.H. Crown Princess Lovisas Foundation, the Professor Nanna Svartz Foundation, and Tore Nilsons Foundation. Work in P. Vanden Berghe’s laboratory is supported by the Research Foundation–Flanders (FWO) (G.0501.10).

Footnotes

Conflict of interest: The authors have declared that no conflict of interest exists.

Citation for this article:J Clin Invest. 2011;121(9):3412–3424. doi:10.1172/JCI58200.

Katarina Sandgren’s present address is: Neurogastroenterology, Department of Experimental Medical Science, Lund University, Lund, Sweden, and Department of Paediatric Surgery, Children’s Hospital Lund, Skåne University Hospital, Malmö, Sweden.

See the related Commentary beginning on page 3386.

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