CSDS enhances the rewarding properties of HFD, consumption of HFD reward pellets, and body weight gain in a ghrelin-dependent manner.
(A) Unlike non-CSDS-exposed, control wild-type mice, CSDS-exposed wild-type mice demonstrate CPP for HFD (**P = 0.021). CSDS-induced HFD reward behavior is not observed in GHSR-null littermates (n = 20 per group). (B) CSDS-exposed wild-type mice gain more weight during the conditioning period than do the other groups (*P = 0.039) (n = 12 per group). (C) CSDS-exposed wild-type mice consume more HFD during CPP conditioning sessions than do non-CSDS-exposed control mice (***P = 0.006); such enhanced HFD intake is not observed in CSDS-exposed GHSR-null mice (n = 12 per group). (D) Consumption of the regular chow pellets available during CPP conditioning sessions is unaffected by CSDS in either genotype (n = 12 per group).