Figure 2
Role of caspases in CD95-, etoposide-, and IR-induced Cer accumulation and apoptosis. Control Jurkat cells (J16; filled bars) or Jurkat cells stably transfected with FLIPL cDNA (JFL2; hatched bars) (34) were treated with anti-CD95 mAb (200 ng/ml) for 6 h, or etoposide (10 μg/ml) or IR (30 Gy) for 16 h. Where indicated (+), cells were pretreated (2 h) and further incubated with 50 μM zVAD-fmk. Cer levels were quantitated and expressed as -fold increase relative to time-matched medium controls. Apoptosis sensitivity of J16 and JFL2 was determined in parallel and expressed as percentage hypoploid cells. Cer data are representative of two independent experiments and show mean ± SD from duplicate samples in one experiment. FLIPL, FLICE-inhibitory protein; mAb, monoclonal antibody; zVAD-fmk, benzoyloxycarbonyl-VAD-fluoromethylketone.
