Figure 1
Shear stress induces the translocation of Cdc42 and Rho. Confluent BAECs were either subjected to a shear stress of 12 dyn/cm2 for various lengths of time as indicated or kept as static controls (represented by time 0). The cell lysates were fractionated into cytosol and membrane fractions. Different fractions were separated by 12.5% SDS-polyacrylamide gel followed by immunoblotting with either a polyclonal anti-Cdc42 or a polyclonal anti-RhoA antibody. Bar graphs represent mean ± SD from three separate experiments. The relative protein amount is defined as the band intensity of the sheared samples (hatched bars) relative to that of the static control (open bars). Asterisks indicate significant difference (P < 0.05) between sheared and static control cells. The bottom of each panel shows G6PDH immunoblotting of the blots from the same lysates indicating that shear stress did not cause the translocation of G6PDH.
