Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein
J. Clin. Invest. Fayez F. Safadi, et al. 103:239 doi:10.1172/JCI5244 [Go to this article.]

Figure 6
Accelerated entry of serum [³H]vitamin D into the liver and its conversion to polar metabolites. [³H]vitamin D₃ was preincubated with aliquots of DBP^+/+ or DBP^–/– serum and injected intravenously into mice in the respective groups. Plasma samples (a) and livers (b) were harvested at the indicated times after injection, and tritium counts were obtained. Data were expressed as a percentage of total cpm injected normalized to total plasma volume (P < 0.05 at 20 and 40 min; a) or per gram of liver (b), and represent the mean ± SEM from three independent experiments. (c) Aliquots of plasma from the 1-min time point in a were extracted and subjected to TLC. The position of a 25(OH)D standard was localized by ultraviolet visualization, and the percentage of total chromatographed cpm migrating in the 25(OH)D region was plotted. (d) The percentage of total chromatographed cpm migrating in the polar region was plotted. The data are the mean ± SEM of two to three independent experiments.