Prenatal nicotine increases pulmonary α7 nicotinic receptor expression and alters fetal lung development in monkeys
J. Clin. Invest. Harmanjatinder S. Sekhon, et al. 103:637 doi:10.1172/JCI5232 [Go to this article.]

Figure 6
(a) Receptor autoradiography showing binding (black) of ¹²⁵I-αBGT to 134-day fetal monkey lung. ×100. Note the similar pattern of ligand binding as immunostaining in Fig. 2a. (b) Confocal image showing binding of Texas red αBGT (red) to cartilaginous airway from control 134-day fetal monkey lung. ×400. Nuclei are counterstained with DAPI (blue). (c) Similar section as in a, but from nicotine-exposed animals, showing strong binding of αBGT to airway epithelial cells and enhanced binding in airway walls. (d) Serial section to c but incubated with Texas red αBGT in the presence of 5 mM nicotine to show specificity of binding. A, airway lumen; V, blood vessel lumen; c, cartilage; αBGT, α-bungarotoxin.