Preservation of KRT15hi hair follicle stem cells in bald scalp, but depletion of CD200hiITGA6hi and CD34hi progenitor cells.
Pseudocolor dot plots (A, B, D, E, G, and H) or overlay graphs (C, F, and I) from FACS analysis of epithelial cells stained with the indicated antibodies. Overlay graphs are plotted first with cells from haired scalp, then layered over with cells from bald scalp. (A–C) Representative example showing KRT15hi cells preserved in bald scalp (n = 8, P = 0.3). In contrast, a defined population of CD200hiITGA6hi cells (D–F; n = 9, P = 0.005) and CD34hi cells (G–I; n = 3, P = 0.011) were depleted in bald scalp. Numerical values represent percent cells in the gated population, of all cells plotted in the representative experiments.