Hepatocyte growth factor leads to recovery from alcohol-induced fatty liver in rats
J. Clin. Invest. Minoru Tahara, et al. 103:313 doi:10.1172/JCI4433 [Go to this article.]

Figure 5
Changes in the rate of apoB synthesis and mobilization of apoB in primary cultured hepatocytes prepared from rat given ethanol diets. Hepatocytes, prepared from rat on ethanol diets for 5 weeks were cultured for 36 h in the absence or presence of 20 ng/ml HGF. Cellular proteins were pulse-labeled with [³⁵S]methionine and [³⁵S]cysteine for 1 h, apoB was immunoprecipitated from the cell lysate, and immune complexes were subjected to SDS-PAGE. For pulse-chase experiments, after cells were pulse-labeled for 1 h, the cells were cultured for 5 h. ApoB was immunoprecipitated from the cell lysate and from the medium, respectively. Immune complexes were visualized by autoradiography. Lower graphs show changes in synthesis and mobilization of ³⁵S-labeled apoB, as determined by densitometric analysis. Each value represents the mean ± SD of triplicate measurements. *P < 0.05, **P < 0.01 vs. control.