Effects of HGF on serum lipid concentrations and hepatic apoB mRNA levels of rats. Changes in serum lipid concentrations (a) (n = 6), lipid compositions in lipoproteins (b) (n = 6), and hepatic apoB mRNA levels (c) (n = 4) are shown. Rats were fed ethanol diets for 37 days, and hrHGF or saline alone was daily injected for the last 7 days during 37 days. (c) Total RNA was dot–blotted to nylon membranes and hybridizes with cDNA for apoB and GAPDH, as described in Methods. In a and c, each value represents the mean ± SD In b, mean values are represented. *P < 0.05, **P < 0.01 vs. saline control. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.