JCI - A mutation in the insulin 2 gene induces diabetes with severe pancreatic β-cell dysfunction in the Mody mouse

A mutation in the insulin 2 gene induces diabetes with severe pancreatic β-cell dysfunction in the Mody mouse
J. Clin. Invest. 103:1 doi:10.1172/JCI4431 [Go to this article.]

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Figure 9

Pulse-chase labeling of CHO cells that express wild-type and mutant insulin. CHO-Ins2^wt (clone w9, upper) and CHO-Ins2^Mody (clone a7, lower) were labeled for 30 min with [³⁵S]methionine. After chasing for indicated times, immunoprecipitation was performed from either cell extracts (C) or media (M), using a mixture of anti-insulin and anti–C-peptide antibodies. Immune complexes were analyzed with tricine–SDS-PAGE (16.5% polyacrylamide gel).