Nucleotide pool imbalance and adenosine deaminase deficiency induce alterations of N-region insertions during V(D)J recombination
J. Clin. Invest. Lisa Gangi-Peterson, et al. 103:833 doi:10.1172/JCI4320 [
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Figure 3Analysis of V(D)J recombinants from Jurkat cells treated with 2′-deoxyguanosine. T cells transfected with either pSJ-F1 or pCJ-F1 were exposed to 100 μM dGuo for 18 h. Sequences of
N-region insertions from 10 illustrative recombinants for each are shown. Comparison of the G + C/A + T ratio from controls with the G + C/A + T ratio from dGuo-treated recombinants was not significant for either construct (
P = 0.68 for pSJ-F1;
P = 0.22 for pCJ-F1). A 2 × 2 contingency table analysis of G–C composition of 37 independent recombinants containing
N-region insertions from signal joints, and 44 independent recombinants containing
N-region insertions from coding joints, is presented. As in Fig.
2, the omission of P nucleotides did not significantly alter the probability calculation. Subtraction of P nucleotides resulted in the following G + C/A + T ratios at
N-region coding joints: control = 1.9; dGuo treated = 2.1. The
P value determined for equivalent
N-region addition to each strand versus no effect, according to the Fisher's exact test, was
P = 0.0001 (signal joint) and
P = 0.70 (coding joint).
