Iris Eke, Ulrike Koch, Stephanie Hehlgans, Veit Sandfort, Fabio Stanchi, Daniel Zips, Michael Baumann, Anna Shevchenko, Christian Pilarsky, Michael Haase, Gustavo B. Baretton, Véronique Calleja, Banafshé Larijani, Reinhard Fässler, Nils Cordes
PINCH1 interacts with PP1α to regulate Akt1 phosphorylation.
(A) Protein phosphatase activity from MEFs left untreated or treated with okadaic acid (OA) or tautomycetin (Tau). Data are mean ± SD (n = 4; **P < 0.01, t test). (B) Protein phosphatase activity of PINCH1 knockdown DLD1 and control cultures. Data are mean ± SD (n = 4; **P < 0.01, t test). (C) Sequence homology search for the putative binding sequence KFVEF. (D) Schematic of mouse PINCH1 WT, PINCH1 V304A/F306A, PINCH1 K302A/V304A/F306A, and PINCH1ΔLIM5 inserted into pEGFP-C1 vector. (E and F) EGFP immunoprecipitates from PINCH1–/– MEF transfected with EGFP, EGFP-PINCH1 WT, V304A/F306A or K302A/V304A/F306A mutated KFVEF motifs, or EGFP-PINCH1ΔLIM5 were examined for total amounts of indicated proteins and protein phosphatase activity (mean ± SD; n = 3; **P < 0.01, t test).