Cell migration from the skin to the DLN in the steady state.
(A) Kaede-Tg mice were photoconverted on the clipped abdominal skin as described in Methods and observed with a fluorescence stereoscopic microscope. Nonphotoconverted clipped skin is shown as a control (middle). Note: nonclipped area remains black since light cannot reach. (B) Skin and draining axillary LN cells resected immediately after violet light exposure of the abdominal skin and resected skin cells not exposed to violet light were subjected to flow cytometric analysis to evaluate the photoconversion. (C and D) Twenty-four hours after photoconversion of the abdominal skin, cells from the draining axillary and other nondraining cervical and popliteal peripheral LNs were stained with CD11c and CD4 mAbs (C) and CD4 and CD44 mAbs (D) and subjected to flow cytometry. These data are representative of at least 5 experiments. Numbers within plots or histograms (B–D) indicate percentage of cells in the respective areas.