Citations to this article


Tregs play an important role in protecting the skin from autoimmune attack. However, the extent of Treg trafficking between the skin and draining lymph nodes (DLNs) is unknown. We set out to investigate this using mice engineered to express the photoconvertible fluorescence protein Kaede, which changes from green to red when exposed to violet light. By exposing the skin of Kaede-transgenic mice to violet light, we were able to label T cells in the periphery under physiological conditions with Kaede-red and demonstrated that both memory phenotype CD4+Foxp3– non-Tregs and CD4+Foxp3+ Tregs migrated from the skin to DLNs in the steady state. During cutaneous immune responses, Tregs constituted the major emigrants and inhibited immune responses more robustly than did LN-resident Tregs. We consistently observed that cutaneous immune responses were prolonged by depletion of endogenous Tregs in vivo. In addition, the circulating Tregs specifically included activated CD25hi Tregs that demonstrated a strong inhibitory function. Together, our results suggest that Tregs in circulation infiltrate the periphery, traffic to DLNs, and then recirculate back to the skin, contributing to the downregulation of cutaneous immune responses.


Michio Tomura ... Osami Kanagawa, Kenji Kabashima


Total citations by year in Crossref

Year: 2014 2013 2012 2011 2010 Total
Citations: 9 8 14 9 2 42
Citation information

Citations to this article in Crossref (42)

Title and authors Publication Year
Natural Killer T Cells Are Essential for the Development of Contact Hypersensitivity in BALB/c Mice
C Shimizuhira, A Otsuka, T Honda, A Kitoh, G Egawa, S Nakajima, C Nakashima, H Watarai, Y Miyachi, K Kabashima
Journal of Investigative Dermatology 2014
Intravital Multiphoton Imaging of Cutaneous Immune Responses
K Kabashima, G Egawa
Journal of Investigative Dermatology 2014
The sympathetic nervous system modulates CD4+Foxp3+ regulatory T cells via noradrenaline-dependent apoptosis in a murine model of lymphoproliferative disease
T Wirth, AM Westendorf, D Bloemker, J Wildmann, H Engler, S Mollerus, M Wadwa, MK Schäfer, M Schedlowski, A Rey
Brain, Behavior, and Immunity 2014
Hapten-Induced Contact Hypersensitivity, Autoimmune Reactions, and Tumor Regression: Plausibility of Mediating Antitumor Immunity
DA Erkes, SR Selvan
Journal of Immunology Research 2014
Biological effects of space radiation and development of effective countermeasures
AR Kennedy
Life Sciences in Space Research 2014
The regulatory T cells induction by epicutaneous immunotherapy is sustained and mediates long-term protection from eosinophilic disorders in peanut-sensitized mice
V Dioszeghy, L Mondoulet, V Dhelft, M Ligouis, E Puteaux, C Dupont, PH Benhamou
Clinical & Experimental Allergy 2014
Chronic Ethanol Feeding Induces Subset Loss and Hyporesponsiveness in Skin T Cells
CP Parlet, TJ Waldschmidt, AJ Schlueter
Alcoholism Clinical and Experimental Research 2014
Endoscopic photoconversion reveals unexpectedly broad leukocyte trafficking to and from the gut
AM Morton, E Sefik, R Upadhyay, R Weissleder, C Benoist, D Mathis
Proceedings of the National Academy of Sciences 2014
Inflammatory lymphangiogenesis: cellular mediators and functional implications
KW Tan, SZ Chong, V Angeli
Angiogenesis 2014
Migration and tolerance: On the road to tolerance - Generation and migration of gut regulatory T cells
O Pabst, G Bernhardt
European Journal of Immunology 2013
Photoconvertible Pathogen Labeling Reveals Nitric Oxide Control of Leishmania major Infection In Vivo via Dampening of Parasite Metabolism
AJ Müller, S Aeschlimann, R Olekhnovitch, M Dacher, GF Späth, P Bousso
Cell Host & Microbe 2013
In Vivo Induction of Cutaneous Inflammation Results in the Accumulation of Extracellular Trap-Forming Neutrophils Expressing RORγt and IL-17
RR Keijsers, AG Hendriks, PE van Erp, B Cranenbroek, PC van de Kerkhof, HJ Koenen, I Joosten
Journal of Investigative Dermatology 2013
Deficiency in IL-17-committed Vγ4+ γδ T cells in a spontaneous Sox13-mutant CD45.1+ congenic mouse substrain provides protection from dermatitis
EE Gray, F Ramírez-Valle, Y Xu, S Wu, Z Wu, KE Karjalainen, JG Cyster
Nature Immunology 2013
New concept of the pathogenesis of atopic dermatitis: Interplay among the barrier, allergy, and pruritus as a trinity
K Kabashima
Journal of Dermatological Science 2013
Epicutaneous Immunity and Onset of Allergic Diseases - Per-"Eczema"tous Sensitization Drives the Allergy March
K Matsumoto, H Saito
Allergology international : official journal of the Japanese Society of Allergology 2013
The inflammatory response of lymphatic endothelium
D Aebischer, M Iolyeva, C Halin
Angiogenesis 2013
Memory T Cell Subsets, Migration Patterns, and Tissue Residence
SN Mueller, T Gebhardt, FR Carbone, WR Heath
Annual Review of Immunology 2013
Lymph node homing of T cells and dendritic cells via afferent lymphatics
R Förster, A Braun, T Worbs
Trends in Immunology 2012
8-Methoxypsoralen plus UVA treatment increases the proportion of CLA+ CD25+ CD4+ T cells in lymph nodes of K5.hTGFβ1 transgenic mice: Letter to the Editor
TP Singh, MP Schön, K Wallbrecht, P Wolf
Experimental Dermatology 2012
The Temporal and Spatial Dynamics of Foxp3+ Treg Cell–Mediated Suppression during Contact Hypersensitivity Responses in a Murine Model
S Lehtimäki, T Savinko, K Lahl, T Sparwasser, H Wolff, A Lauerma, H Alenius, N Fyhrquist
Journal of Investigative Dermatology 2012
Regulatory T Cells in Atherogenesis
N Sasaki, T Yamashita, M Takeda, K Hirata
Journal of Atherosclerosis and Thrombosis 2012
Immunoregulation of skin sensitization and regulatory T cells
I Kimber, MA Travis, SF Martin, RJ Dearman
Contact Dermatitis 2012
Immunoregulation in cutaneous allergy: prevention and control
CE Zielinski, T Zuberbier, M Maurer
Current Opinion in Allergy and Clinical Immunology 2012
Update of Immune Events in the Murine Contact Hypersensitivity Model: Toward the Understanding of Allergic Contact Dermatitis
T Honda, G Egawa, S Grabbe, K Kabashima
Journal of Investigative Dermatology 2012
Dissection of a Circulating and Intrahepatic CD4+Foxp3+ T-Cell Subpopulation in Chronic Hepatitis B Virus (HBV) Infection: A Highly Informative Strategy for Distinguishing Chronic HBV Infection States
M Zhang, J Zhou, T Zhao, G Huang, Y Tan, S Tan, X Fu, W Niu, G Meng, X Chen, X Shang, D Liu, B Ni, L Wang, Y Wu
The Journal of Infectious Diseases 2012