JCI - Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling

Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling

Stefanie Eyerich, Kilian Eyerich, Davide Pennino, Teresa Carbone, Francesca Nasorri, Sabatino Pallotta, Francesca Cianfarani, Teresa Odorisio, Claudia Traidl-Hoffmann, Heidrun Behrendt, Stephen R. Durham, Carsten B. Schmidt-Weber, Andrea Cavani

Published in Volume 119, Issue 12

J Clin Invest. 2009; 119(12):3573–3585 doi:10.1172/JCI40202

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Figure 1

Th22 cells represent a distinct T cell subset enriched in inflammatory skin diseases.

(A–D) Representative 4-color intracellular cytokine stainings of primary human T lymphocytes derived from PBMCs (A), PS (B), AE (C), and ACD (D) for IL-22, IL-17, IFN-γ, and IL-4. Shown are dot plots for IL-22 and IL-17 and of IL-17^–IL-22⁺ gated cells (red outline) for IFN-γ and IL-4. Th22 cells are shown by red shading. Numbers indicate relative percentages per quadrant. (E) Frequency of IL-22⁺ cells in PBMCs (n = 4) and in PS (n = 3), AE (n = 4), and ACD (n = 3). Symbols denote individual determinations, and horizontal bars denote mean. *P < 0.05; **P < 0.01. (F) Plasticity in T cell phenotypes, as shown by coexpression of IL-22 with other cytokines in PS (n = 3), AE (n = 4), and ACD (n = 4). Gray ovals denote scale, indicating 1% of total cells. (G) The majority of IL-22⁺ T cells belongs to the CD4⁺ subpopulation, as shown for CD4/CD8 sorted cells from PBMCs and skin T cell lines of PS, AE, and ACD. Numbers indicate relative percentages per quadrant.