Figure 5
Vascular remodeling during development of wound-induced de novo AVM.
A light-microscopic view and the corresponding Hb(O2) pseudocolor map of vascular morphogenesis during wound healing for 8 days in the mutant. Arteries are readily distinguishable from veins by high O2 levels days 1–5 after wounding (A–D). Early stages of AV shunts (dotted circles in B and D) were detected as early as day 3. White arrows in B, C, and E indicate veins containing high Hb(O2) levels. Blue arrows in C and F indicate veins at downstream of the AV shunts, showing dilated vascular morphologies. By day 6 (E–G), Hb(O2) levels in the veins draining from the AV shunts are as high as those in the arteries feeding to AV shunts. It is noteworthy that there were signs of bleeding where AV shunts are established (red arrows in B–D). Ratio of Hb(O2) saturation of veins and arteries in the mutants was elevated as wound healing progressed, while that in control mice remained unchanged (H). Error bars in H indicate SD. Scale bar in A: 1 mm.
