Figure 3
Spirochetes shed myriad blebs during early feeding (24 hours after placement).
(A) Composite confocal image showing the distribution of spirochetes and numerous blebs through the full thickness of a midgut (45 μm); shown in the inset is a spirochete shedding blebs. Arrow indicates the cell in the consecutive confocal sections in B–H. (B–H) Localization of an intracellular bleb (arrow) present within A by consecutive 0.5-μm confocal sections acquired through the full depth (3.5 μm) of an epithelial cell. N, nucleus. Scale bars: 25 μm.